Bioprocess and Biosystems Engineering

, Volume 42, Issue 4, pp 593–602 | Cite as

Role of media composition in biomass and astaxanthin production of Haematococcus pluvialis under two-stage cultivation

  • Yongteng Zhao
  • Chenchen Yue
  • Shuxiang Geng
  • Delu Ning
  • Ting Ma
  • Xuya YuEmail author
Research Paper


In the present study, the effects of four different culture media on the growth, astaxanthin production and morphology of Haematococcus pluvialis LUGU were studied under two-step cultivation. The interactions between astaxanthin synthesis and secondary messengers, reactive oxygen species (ROS) and mitogen-activated protein kinases (MAPK) were also investigated. In the first green vegetative cell stage, maximal biomass productivity (86.54 mg L−1 day−1) was obtained in BBM medium. In the induction stage, the highest astaxanthin content (21.5 mg g−1) occurred in BG-11 medium, which was higher than in any other media. The expressions of MAPK and astaxanthin biosynthetic genes in BG-11 were higher than in any other media, whereas the ROS content was lower. Biochemical and physiological analyses suggested that the ROS, MAPK and astaxanthin biosynthetic gene expression was involved in astaxanthin biosynthesis in H. pluvialis under different culture media conditions. This study proposes a two-step cultivation strategy to efficiently produce astaxanthin using microalgae.


Haematococcus pluvialis Astaxanthin Two-step cultivation Reactive oxygen species Mitogen-activated protein kinases 



The National Natural Science Foundation of China (no. 21766012 to X. Yu), the Key Science and Technology Project of Yunnan Province, China (2018ZG003 to X. Yu), and the National Natural Science Foundation of China (no. 21666012 to P. Zhao) supported this study.

Supplementary material

449_2018_2064_MOESM1_ESM.docx (16 kb)
Supplementary material 1 (DOCX 15 KB)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yongteng Zhao
    • 1
  • Chenchen Yue
    • 1
  • Shuxiang Geng
    • 2
  • Delu Ning
    • 2
  • Ting Ma
    • 2
  • Xuya Yu
    • 1
    Email author
  1. 1.Faculty of Life Science and TechnologyKunming University of Science and TechnologyKunmingChina
  2. 2.Yunnan Academy of ForestryKunmingChina

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