Rational high-throughput system for screening of high sophorolipids-producing strains of Candida bombicola
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A rational high-throughput screening can significantly improve the efficiency of strain screening with high performance. In this study, based on the addition reaction of unsaturated fatty acids in the sophorolipids (SLs) and I2 molecules, a simple and rapid high-throughput detection method for SLs was established which demonstrated a correlation coefficient (R2) of 0.9106 with high-performance liquid chromatography (HPLC) method. Moreover, chlorpromazine, as a rational selecting pressure for enrichment of mutants with high cytochrome P450 enzyme activity, which was a key enzyme in the synthesis of SLs, was introduced into the high-throughput screening model. Consequently, with the aid of this effective screening system, a high-yielding mutant designated as Candida bombicola F6.5 was successfully screened out from 1500 single colonies, which presented improvements of 40.3% and 11.4% on SLs titer and yield, respectively, compared to the parent strain in a 1 L bioreactor.
A simple and rapid high-throughput SLs-detecting method was established.
The chlorpromazine as a rational selecting pressure was introduced.
A high SLs-producing mutant was obtained by high-throughput screening technology.
40.3% and 11.4% improvements in SLs titer and yield were achieved.
KeywordsSophorolipids Mutation High-throughput screening Chlorpromazine
This work was financially supported by the National Key Research and Development Program (2017YFB0309302), the Major State Basic Research Development Program of China (973 Program, No. 2012CB721006) and the Fundamental Research Funds for the Central Universities (WF1814032, 22221817014, 22221818014).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
- 2.Kim K (2002) Characteristics of sophorolipid as an antimicrobial agent. J Microbiol Biotechnol 12(2):235–241Google Scholar
- 4.Oliveira MRD, Magri A, Baldo C, Camilios-Neto D, Minucelli T, Antonia M (2015) Review: sophorolipids a promising biosurfactant and it’s applications. Int J Adv Biotechnol Res 6:161–174Google Scholar
- 12.Zhong GQ (2004) Study on determination method of Iodine value of oil and fat. Sci Technol Cereals Oils Foods 12(1):29–30Google Scholar
- 17.Jiao P, Ma S, Huang Y, Hu P, Cao Z (2000) Two-step enrichment and double-time replica plating technology for Candida tropicalis mutant selection. J Tsinghua Univ 40(6):22–25Google Scholar