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Overproduction of laccase from a newly isolated Ganoderma lucidum using the municipal food waste as main carbon and nitrogen supplement

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Abstract

A strain of Ganoderma lucidum was separated and identified according to its morphological characteristics and phylogenetic data. The fungus is a laccase producer and it can secrete laccase using the municipal food waste (FW) as carbon and nitrogen supplement. After the statistic optimization, a laccase activity of 42,000 ± 600 U/l was obtained at 500 ml flask level and the activity is 12,000 U/l higher than that obtained by fermenting glucose and peptone, indicating that the use of FW to produce laccase not only reduces production cost, but also improves laccase activity. In 15 l bioreactor, FW is also suitable for laccase production and the maximum laccase activity reached 54,000 U/l. Moreover, some details of laccase overproduction using FW were investigated. The G. lucidum consumes FW by secreting a series of hydrolases and proteases and the improvement of laccase activity is because FW induces over-expression of three isoenzymes by polyacrylamide gel electrophoresis analysis.

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Acknowledgments

This work was supported by National Science Foundation of China (No. 51008119), and the fund for key subject of ecology, Henan Normal University, China.

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Authors and Affiliations

  1. College of Life Sciences, Henan Normal University, Xinxiang, 453007, China

    Wang Hailei, Li Ping, Yang Yuhua & Liu Yufeng

  2. Key Laboratory for Microorganisms and Functional Molecules, University of Henan Province, Xinxiang, 453007, China

    Wang Hailei

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  1. Wang Hailei
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  2. Li Ping
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  3. Yang Yuhua
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  4. Liu Yufeng
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Correspondence to Wang Hailei.

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Hailei, W., Ping, L., Yuhua, Y. et al. Overproduction of laccase from a newly isolated Ganoderma lucidum using the municipal food waste as main carbon and nitrogen supplement. Bioprocess Biosyst Eng 38, 957–966 (2015). https://doi.org/10.1007/s00449-014-1341-z

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  • DOI: https://doi.org/10.1007/s00449-014-1341-z

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