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Effect of organic acids found in cottonseed hull hydrolysate on the xylitol fermentation by Candida tropicalis

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Abstract

Five organic acids (acetic, ferulic, 4-hydroxybenzoic, formic and levulinic acids) typically associated in the hemicellulose hydrolysate were selected to study their effects on the xylitol fermentation. The effects of individual and combined additions were independently evaluated on the following parameters: inhibitory concentration; initial cell concentration; pH value; and membrane integrity. The results showed that the toxicities of organic acids were related to their hydrophobility and significantly affected by the fermentative pH value. In addition, it was revealed that the paired combinations of organic acids did not impose synergetic inhibition. Moreover, it was found that the fermentation inhibition could be alleviated with the simple manipulations by increasing the initial cell concentration, raising the initial pH value and minimizing furfural levels by evaporation during the concentration of hydrolysates. The proposed strategies for minimizing the negative effects could be adopted to improve the xylitol fermentation in the industrial applications.

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Acknowledgments

We are indebted to the National High-tech Research and Development Program (2011AA02A207) and to the Natural Science Foundation of China (20976009, 21176018) for their generous financial supports.

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Authors and Affiliations

  1. College of Life Science and Technology, Beijing University of Chemical Technology, No. 15 North Third Ring East Road, Chaoyang District, 100029, Beijing, China

    Le Wang, Pingwah Tang & Qipeng Yuan

  2. College of Bioengineering, Henan University of Technology, 450000, Zhengzhou, China

    Le Wang

  3. College of Chemistry and Chemical Engineering, Henan Normal University, 453002, Xinxiang, China

    Dapeng Wu

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  1. Le Wang
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Correspondence to Qipeng Yuan.

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Wang, L., Wu, D., Tang, P. et al. Effect of organic acids found in cottonseed hull hydrolysate on the xylitol fermentation by Candida tropicalis . Bioprocess Biosyst Eng 36, 1053–1061 (2013). https://doi.org/10.1007/s00449-012-0858-2

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  • DOI: https://doi.org/10.1007/s00449-012-0858-2

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