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Immunolocalization and changes of 17beta-estradiol during ovarian development of Chinese mitten crab Eriocheir Sinensis

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Abstract

17beta-estradiol (E2) is important for crustacean ovarian development. This study aims to investigate the distribution and change pattern of E2 in the ovary, hepatopancreas, thoracic ganglion and brain ganglion as well as Vg-mRNA expression level during ovarian development of Chinese mitten crab Eriocheir sinensis. Results showed that strongly positive signals of E2 were mainly distributed in follicle cells of ovaries for all developmental stages as well as oocyte cytoplasm of stages III to V ovaries. In hepatopancreas, the E2-positive signal was mainly detected in the cytoplasm and nucleus of fibrillar cells and the nucleus of resorptive cells, while the maximum fluorescence intensity was observed in stage III hepatopancreas. On the contrary, the E2 immunoreactivities in nervous tissues were relatively stable during ovarian development. Moreover, the changing pattern of E2 concentration was similar within hemolymph, ovary and hepatopancreas during the ovarian development. From stages I to III, the E2 content in three tissues increased significantly, then decreased gradually until stage V. As for the Vg-mRNA expression level in hepatopancreas and ovaries, an increasing trend was found in ovaries but no significant difference was detected during the period of ovarian stages III to V. Hepatopancreatic Vg-mRNA expression level increased significantly during stages I to IV and dramatically decreased at stage V. In conclusion, our study suggests that ovary, hepatopancreas, hemolymph and nervous tissues are the target organs of E2 in E. sinensis and E2 concentrations in different tissues are closely related to vitellogenesis in ovary and hepatopancreas during ovarian development.

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  • 18 June 2018

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Acknowledgements

This work was supported by the general project [grant number 31572630] from the Natural Science Foundation of China, the Capacity Promotion Projects [grant numbers 16DZ2281200, 13DZ2280500] of Shanghai Engineering and Technology Center from Shanghai Municipal Science and Technology Commission and the extension project [grant number 2015-1-7] from Shanghai Agriculture Committee. Infrastructure costs were supported by the Shanghai Universities Top Disciplines Project [grant number 2015-62-0908] of Fisheries from Shanghai Municipal Education Committee and the Special Fund [grant number CARS-46] of Modern Agricultural Technology System-47 from Ministry of Agriculture of China.

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  1. Jie Pan and Meimei Liu contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China

    Jie Pan, Meimei Liu, Yongxu Cheng & Xugan Wu

  2. Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China

    Yongxu Cheng & Xugan Wu

  3. National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China

    Yongxu Cheng & Xugan Wu

  4. The Key Laboratory of Applied Marine Biology of Guangdong Province and Chinese Academy of Sciences, South China Sea Institute of Oceanology, CAS, Guangzhou, 510301, China

    Ting Chen

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Pan, J., Liu, M., Chen, T. et al. Immunolocalization and changes of 17beta-estradiol during ovarian development of Chinese mitten crab Eriocheir Sinensis. Cell Tissue Res 373, 509–520 (2018). https://doi.org/10.1007/s00441-018-2834-x

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