Distribution and chemical coding of cocaine- and amphetamine-regulated transcript peptide (CART)-immunoreactive neurons in the guinea pig bowel

Abstract.

Cocaine- and amphetamine-regulated transcript-immunoreactive (CART-IR) neurons and nerve fibers were abundant in the submucosal and myenteric plexuses of the guinea pig duodenum, ileum, cecum, proximal and distal colon. CART immunoreactivity was also observed in cell bodies and nerve fibers in the extrahepatic biliary tract. In the myenteric plexus, similar proportions (~20–25%) of neurons were CART-IR in all regions, with the exception of the cecum, where only 13% were CART-IR. In the submucosal plexus, CART-IR was detected in 35–50% of the neurons along the bowel with the exception of the proximal colon (~10%). Multiple label immunohistochemistry in the myenteric plexus of the ileum demonstrated that CART-IR neurons were also immunoreactive for choline acetyltransferase (83%), tachykinins (77%), calbindin (50%), nitric oxide synthase (20%), and/or vasoactive intestinal peptide (23%). In triple label studies, we found that ~8% of the CART-IR neurons were also immunoreactive for both choline acetyltransferase and nitric oxide synthase. CART immunoreactivity was not colocalized with calretinin, somatostatin, or serotonin. These results, combined with previous studies of chemical coding and projection patterns in the guinea pig ileum, indicate that at least four different classes of gut neurons in the myenteric plexus express CART peptide, including excitatory and inhibitory motor neurons projecting to the circular muscle, intrinsic primary afferent neurons, and a subset of descending interneurons. Because all CART-IR neurons in the submucosal plexus were also vasoactive intestinal peptide-IR, they are likely to include secretomotor/vasodilator neurons.

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References

  1. Brookes SJ (2001) Classes of enteric nerve cells in the guinea-pig small intestine. Anat Rec 262:58–70

    Article  CAS  PubMed  Google Scholar 

  2. Brookes SJH, Steele PA, Costa M (1991) Calretinin immunoreactivity in cholinergic motor neurones, interneurones and vasomotor neurones in the guinea pig small intestine. Cell Tissue Res 263:471–481

    CAS  PubMed  Google Scholar 

  3. Brookes SJH, Steele PA, Costa M (1991) Identification and immunohistochemistry of cholinergic and non-cholinergic circular muscle motor neurons in the guinea-pig small intestine. Neuroscience 42:863–878

    CAS  PubMed  Google Scholar 

  4. Brookes SJ, Song ZM, Steele PA, Costa M (1992) Identification of motor neurons to the longitudinal muscle of the guinea pig ileum. Gastroenterology 103:961–973

    CAS  PubMed  Google Scholar 

  5. Buchan AM, Sikora LK, Levy JG, McIntosh CH, Dyck I, Brown JC (1985) An immunocytochemical investigation with monoclonal antibodies to somatostatin. Histochemistry 83:175–180

    CAS  PubMed  Google Scholar 

  6. Costa M, Furness JB, Pompolo S, Brookes SJH, Bornstein JC, Bredt DS, Snyder SH (1992) Projections and chemical coding of neurons with immunoreactivity for nitric oxide synthase in the guinea-pig small intestine. Neurosci Lett 148:121–125

    CAS  PubMed  Google Scholar 

  7. Costa M, Brookes SJ, Steele PA, Gibbins I, Burcher E, Kandiah CJ (1996) Neurochemical classification of myenteric neurons in the guinea-pig ileum. Neuroscience 75:949–967

    Article  CAS  PubMed  Google Scholar 

  8. Couceyro P, Paquet M, Koylu E, Kuhar MJ, Smith Y (1998) Cocaine- and amphetamine-regulated transcript (CART) peptide immunoreactivity in myenteric plexus neurons of the rat ileum and co-localization with choline acetyltransferase. Synapse 30:1–8

    Article  CAS  PubMed  Google Scholar 

  9. Douglass J, McKinzie AA, Couceyro P (1995) PCR differential display identifies a rat brain mRNA that is transcriptionally regulated by cocaine and amphetamine. J Neurosci 15:2471–2481

    CAS  PubMed  Google Scholar 

  10. Furness J, Clerc N, Gola M, Kunze W, Fletcher E (2000) Identification of component neurons and organisation of enteric nerve circuits. In: Krammer H, Singer M (eds) Neurogastroenterology from the basics to the clinics. Kluwer Academic, Dordrecht, pp 137–150

  11. Gershon MD, Kirchgessner AL, Wade PR (1994) Functional anatomy of the enteric nervous system. In: Johnson LR (ed) Physiology of the gastrointestinal tract. Raven, New York, pp 381–422

  12. Kristensen P, Judge ME, Thim L, Ribel U, Christjansen KN, Wulff BS, Clausen JT, Jensen PB, Madsen OD, Vrang N, Larsen PJ, Hastrup S (1998) Hypothalamic CART is a new anorectic peptide regulated by leptin. Nature 393:72–76

    CAS  PubMed  Google Scholar 

  13. Kuhar MJ, Dall Vechia SE (1999) CART peptides: novel addiction- and feeding-related neuropeptides. Trends Neurosci 22:316–320

    CAS  PubMed  Google Scholar 

  14. Kunze WAA, Bornstein JC, Furness JB (1995) Identification of sensory nerve cells in a peripheral organ (the intestine) of a mammal. Neuroscience 66:1–4

    Article  CAS  PubMed  Google Scholar 

  15. Larsen PJ, Vrang N, Petersen PC, Kristensen P (2000) Chronic intracerebroventricular administration of recombinant CART(42–89) peptide inhibits and causes weight loss in lean and obese Zucker (fa/fa) rats. Obes Res 8:590–596

    CAS  PubMed  Google Scholar 

  16. Lin Z, Gao N, Hu HZ, Liu S, Gao C, Kim G, Ren J, Xia Y, Peck OC, Wood JD (2002) Immunoreactivity of Hu proteins facilitates identification of myenteric neurones in guinea-pig small intestine. Neurogastroenterol Motil 14:197–204

    CAS  PubMed  Google Scholar 

  17. Mawe GM, Ellis LM (2001) Chemical coding of intrinsic and extrinsic nerves in the guinea pig gallbladder: distributions of PACAP and orphanin FQ. Anat Rec 262:101–109

    Article  CAS  PubMed  Google Scholar 

  18. O'Donnell AM, Ellis LM, Riedl MS, Elde RP, Mawe GM (2001) Distribution and chemical coding of orphanin FQ/nociceptin-immunoreactive neurons in the myenteric plexus of guinea pig intestines and sphincter of Oddi. J Comp Neurol 430:1–11

    Article  CAS  PubMed  Google Scholar 

  19. Portbury AL, Pompolo S, Furness JB, Stebbing MJ, Kunze WA, Bornstein JC, Hughes S (1995) Cholinergic, somatostatin-immunoreactive interneurons in the guinea pig intestine: morphology, ultrastructure, connections and projections. J Anat 187:303–321

    Google Scholar 

  20. Song Z-M, Brookes SHJ, Costa M (1994) All calbindin-immunoreactive myenteric neurons project to the mucosa of guinea-pig small intestine. Neurosci Lett 180:219–222

    CAS  Google Scholar 

  21. Song ZM, Brookes SJ, Ramsay GA, Costa M (1997) Characterization of myenteric interneurons with somatostatin immunoreactivity in the guinea-pig small intestine. Neuroscience 80:907–923

    Article  CAS  PubMed  Google Scholar 

  22. Steele PA, Brookes SJ, Costa M (1991) Immunohistochemical identification of cholinergic neurons in the myenteric plexus of guinea-pig small intestine. Neuroscience 45:227–239

    CAS  PubMed  Google Scholar 

  23. Timmermans JP, Adriaensen D, Cornelissen W, Scheuermann DW (1997) Structural organization and neuropeptide distribution in the mammalian enteric nervous system, with special attention to those components involved in mucosal reflexes. Comp Biochem Physiol A Physiol 118:331–340

    Article  CAS  PubMed  Google Scholar 

  24. Young HM, Furness JB (1995) Ultrastructural examination of the targets of serotonin-immunoreactive descending interneurons in the guinea pig small intestine. J Comp Neurol 356:101–114

    CAS  PubMed  Google Scholar 

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Acknowledgements.

We are grateful to the CURE/UCLA/DDC Antibody/RIA Core Laboratory (DK41301) for the rabbit and mouse anti-VIP antisera. Also, we wish to thank Dr. David Linden for his assistance.

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Correspondence to Gary M. Mawe.

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This work was supported by NIH grants NS26995, DK62267 and DK45410

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Ellis, L.M., Mawe, G.M. Distribution and chemical coding of cocaine- and amphetamine-regulated transcript peptide (CART)-immunoreactive neurons in the guinea pig bowel. Cell Tissue Res 312, 265–274 (2003). https://doi.org/10.1007/s00441-002-0678-9

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Keywords

  • Autonomic nervous system
  • Innervation
  • Gut peptides
  • Enteric nervous system
  • Myenteric plexus
  • Submucosal plexus
  • Guinea pig