The miR-17-92 cluster has been involved in the cell cycle, apoptosis, and signaling. However, its transcriptional regulation has not been fully characterized. To elucidate the transcriptional regulation, the promoter of miR-17-92 was analyzed in detail in pig here. We found that, as an intronic miRNA, porcine miR-17-92 cluster was regulated by two independent promoters, an A/T-rich region directly upstream of the miR-17-92 coding sequence, and a G/C-rich region corresponding to the host gene promoter of the human miR-17-92 cluster. Several cis-regulatory elements were identified including sites for c-Myc, NFY, E2F3, and SP1, among which NFY and c-Myc sites were present in both A/T- and G/C-rich regions, while E2F3 and SP1 sites only existed in G/C-rich region. Sites for c-Myc, E2F3, and SP1 were positive for regulating transcription. NFY sites played bipartite roles, functioning as a repressor for the A/T-rich region, and as an activator for the G/C-rich region. Additionally, we found that levels of individual miRNAs in the cluster were not promoted completely in parallel with each other or with pri-miR-17-92 by the A/T-rich region, through using a self-made vector by modifying pGL3-basic in which firefly luciferase gene was replaced with an miR-17-92 cluster and a direct upstream A/T-rich region. The expression regulation of miR-17-92 is complicated and the results will contribute to further revealing the regulatory mechanisms under the expression of the miR-17-92 cluster.
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This work was supported by the National Natural Science Foundation of China (31741114), Foundation for Improving Innovative Capability of Scientific Institutions, Heilongjiang (YC2016D001), and National Science and technology Planning Project of “12th Five-Year” in Rural Areas (2015BAD03B02-5).
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Communicated by S. Hohmann.
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Yang, X., Zhang, C., Wang, J. et al. Transcriptional regulation of the porcine miR-17-92 cluster. Mol Genet Genomics 294, 1023–1036 (2019). https://doi.org/10.1007/s00438-019-01560-0
- miR-17-92 cluster
- cis-regulatory element
- Transcription factor