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Characterization of SBEIIa homoeologous genes in bread wheat

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Abstract

To elucidate some of the molecular mechanisms involved in genome differentiation and evolution of cultivated wheats, we compared orthologous genes encoding starch branching enzyme IIa (SBEIIa). Bread wheat is an allohexaploid species comprising the three genomes A, B and D, each of which contributes a copy of the SBEIIa gene, involved in starch biosynthesis and known to control important quality traits related to technological and nutritional value of wheat-based food products. Alignment of the nucleotide sequences of these three genes revealed variation, both at the level of single nucleotides and indels. Multiple transposon elements were identified in the intragenic regions, some of which appear to have inserted before the divergence of the wheat diploid genomes. The B genome homoeologue was the most divergent of the three genes. Two MITE transposon insertions were detected within the intronic sequence of SBEIIa-B and two other transposons within SBEIIa-D. The presence/absence of these transposons in a panel of diploid and polyploid Triticum and Aegilops species provided some insights into the phylogeny of wheat.

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Authors and Affiliations

  1. Department of Agriculture, Forestry, Nature and Energy, University of Tuscia, 01100, Viterbo, Italy

    E. Botticella, F. Sestili & D. Lafiandra

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  1. E. Botticella
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  2. F. Sestili
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  3. D. Lafiandra
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Correspondence to D. Lafiandra.

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Communicated by S. Hohmann.

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Botticella, E., Sestili, F. & Lafiandra, D. Characterization of SBEIIa homoeologous genes in bread wheat. Mol Genet Genomics 287, 515–524 (2012). https://doi.org/10.1007/s00438-012-0694-8

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