Architecture of the nervous system in metacercariae of Diplostomum pseudospathaceum Niewiadomska, 1984 (Digenea)

Abstract

The development of metacercariae of Diplostomum pseudospathaceum Niewiadomska, 1984 is accompanied by profound morphological transformations often characterized as metamorphosis, which makes these metacercariae an interesting case for studying the morphogenesis of the digenean nervous system. Although the nervous system of D. pseudospathaceum is one of the most extensively studied among digeneans, there are still gaps in our knowledge regarding the distribution patterns of some neuroactive substances, most notably neuropeptides. The present study addresses these gaps by studying pre-infective metacercariae of D. pseudospathaceum using immunochemical staining and confocal microscopy to characterize the distribution patterns of serotonin (5-HT) and two major groups of flatworm neuropeptides, FMRFamide-related (FaRPs) and substance P-related (SP) peptides. The general morphology of the nervous system was examined with antibodies to alpha-tubulin. The nervous system of the metacercariae was shown to conform to the most common morphology of the nervous system in the hermaphroditic generation, with three pairs of posterior nerve cords and four pairs of anterior nerves. The patterns of FaRP- and 5-HT immunoreactivity (IR) were similar to those revealed in earlier studies by cholinesterase activity, which is in accordance with the known role of these neurotransmitters in controlling muscle activity in flatworms. The SP-IR nervous system was significantly different and consisted of mostly bipolar cells presumably acting as mechanoreceptors. The architecture of the nervous system in D. pseudospathaceum metacercariae is discussed in comparison to that in cercariae of D. pseudospathaceum and metacercariae of related digenean species.

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Acknowledgements

Confocal microscopy was performed at the “Taxon” Research Resource Centre (Zoological Institute, Russian Academy of Sciences) (http://www.ckp-rf.ru/ckp/3038/?sphrase_id=8879024).

Funding

This work was supported by budget funding (projects АААА-А17-117030110029-3 and АААА-А17-117030310322-3) of the Russian Academy of Sciences and by the Russian Foundation for Basic Research (grant 16-04-00593).

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Petrov, A.A., Podvyaznaya, I.M. & Zaitseva, O.V. Architecture of the nervous system in metacercariae of Diplostomum pseudospathaceum Niewiadomska, 1984 (Digenea). Parasitol Res 118, 1193–1203 (2019). https://doi.org/10.1007/s00436-019-06231-y

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Keywords

  • Nervous system
  • Metacercariae
  • 5-HT
  • Substance P
  • FMRFamide
  • Alpha-tubulin