Skip to main content
SpringerLink
Log in

Acaricidal properties of the formulations based on essential oils from Cymbopogon winterianus and Syzygium aromaticum plants

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

The cattle tick, Rhipicephalus (Boophilus) microplus, has caused serious harm to livestock raising in Brazil, considering the costs of controlling it, loss of revenue due to smaller production of milk and meat, and damage to leather, in addition to transmitting diseases. The use of medicinal plants is considered an alternative to the recurring resistance to chemicals. Due to the need for efficient alternatives with less environmental impact, this study aimed to develop contact formulations with essential oils from the Java citronella (Cymbopogon winterianus) and clove (Syzygium aromaticum) plants and to assess in vitro the effects in different stages of the tick cycle. In the present study, concentrations from 0.5–15.0 % of the essential oils incorporated in the formulations were used. The ticks from different geographical areas were treated with those formulations, and their effects on the production levels of eggs, on the larvae hatching, and their efficiency on ticks were assessed. The obtained results were compared with other commercial acaricidal products. After the 20th day of treatment, the formulations with citronella essential oil had 2.09–55.51 % efficiency, depending on the concentration of the oil incorporated. The efficiency of the treatment with formulations containing clove essential oil was higher, from 92.47–100 %. The results showed the acaricidal effects of the formulations tested when compared to commercial chemical products. In vivo studies should be performed in order to assess the efficiency of those formulations in the fields, aiming to use these products as an alternative for controlling cattle ticks.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Affonso RS, Rennó MN, Slana GBCA, França TCC (2012) Aspectos Químicos e Biológicos do Óleo Essencial de Cravo da Índia. Rev Virtual Quim 4(2):146–161

    CAS  Google Scholar 

  • Agnolin CA, Olivo CJ, Leal MLR, Beck RCR, Meinerz GR, Parra CLC, Machado PR, Foletto V, Bem CM, Nicolodi PRSJ (2010) Eficácia do óleo de citronela [Cymbopogon nardus (L.) Rendle] no controle de ectoparasitas de bovinos. Rev Bras Plantas Med 12(4):482–487. doi:10.1590/s1516-05722010000400012

    Article  Google Scholar 

  • Álvarez V, Loiaza J, Bonilla R, Barrios M (2008) Control in vitro de garrapatas (Boophilus microplus; Acari: Ixodidae) mediante extractos vegetales. Rev Biol Trop 56(1):291–302

    PubMed  Google Scholar 

  • Alves WV, Lorenzetti ER, Gonçalves FC (2012) Utilização de acaricidas a base de plantas no controle de Rhipicephalus (Boophilus) microplus: uma contribuição para a produção e desenvolvimento sustentável. Rev Bras Agrop Sustent 2(2):14–25

    Google Scholar 

  • Ansel HC, Popovich NG, Allen LV Jr (2000) Formas farmacêuticas e sistemas de liberação de fármacos Premier, São Paulo

    Google Scholar 

  • Brasil (1997) Portaria 48. de 12 de maio de 1997. MAPA, Brasília http://sistemasweb.agricultura.gov.br/sislegis/action/detalhaAto.do?method=visualizarAtoPortalMapa&chave=72818869. Acessed 23 July 2014

  • Brasil (2004) Guia de Estabilidade de Produtos Cosméticos. ANVISA, Brasília http://www.anvisa.gov.br/divulga/public/series/cosmeticos.pdf. Acessed 20 March 2014

  • Brasil (2012) Produção da Pecuária Municipal. IBGE, Rio de Janeiro ftp://ftp.ibge.gov.br/Producao_Pecuaria/Producao_da_Pecuaria_Municipal/2012/ppm2012.pdf. Acessed 01 August 2014

  • Cassel E, Vargas RMF (2006) Experiments and modeling of the Cymbopogon winterianus essential oil extraction by steam distillation. J Mex Chem Soc 50(3):126–129

    CAS  Google Scholar 

  • Chagas ACS (2004) Controle de parasitas utilizando extratos vegetais. Rev Bras Parasitol Vet 13:156–160

    Google Scholar 

  • Chagas ACS, Passos WM, Prates HT, Leite RC, Furlong J, Fortes ICP (2002) Efeito acaricida de óleos essenciais e concentrados emulsionáveis de Eucalyptus spp em Boophilus microplus. Braz J Vet Res Anim Sci 39(5):247–253. doi:10.1590/S1413-95962002000500006

    Article  Google Scholar 

  • Chagas ACS, Barros LD, Cotinguiba F, Furlan M, Giglioti R, Oliveira MCS, Bizzo HR (2012) In vitro efficacy of plant extracts and synthesized substances on Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). Parasitol Res 110:295–303. doi:10.1007/s00436-011-2488-z

    Article  Google Scholar 

  • Chungsamarnyart N, Jiwajinda S, Rattanakreetakul C, Jansawan W (1991) Practical extraction of sugar apple seeds against tropical cattle ticks. Kasetsart J Nat Sci Suppl 25:101–105

    Google Scholar 

  • Drummond RO, Ernst SE, Trevino JL, Gladney WJ, Graham OH (1973) Boophilus annulatus and B. microplus: laboratory tests of insecticides. Agric Res Serv 66(1):130–133

    CAS  Google Scholar 

  • Graf JF, Gogolewski R, Leach-Bing N, Sabatini GA, Molento MB, Bordin EL, Arantes GJ (2004) Tick control: an industry point of view. Parasitology 129:427–442. doi:10.1017/s0031182004006079

    Article  Google Scholar 

  • Grisi L, Leite RC, Martins JRS, Barros ATM, Barros ATM, Andreotti R, Cançado PHD, León AAP, Pereira JB, Villela HS (2014) Reassessment of the potential economic impact of cattle parasites in Brazil. Braz J Vet Parasitology 23(2):150–156. doi:10.1590/s1984-29612014042

    Article  Google Scholar 

  • Martinez-Velazquez M, Rosario-Cruz R, Castillo-Herrera G, Flores-Fernandez JM, Alvarez AH, Lugo-Cervantes E (2011) Acaricidal effect of essential oils from Lippia graveolens (Lamiales: Verbenaceae), Rosmarinus officinalis (Lamiales: Lamiaceae), and Allium sativum (Liliales: Liliaceae) against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae). J Med Entomol 48(4):822–827

    Article  CAS  PubMed  Google Scholar 

  • Martins RM (2006) Estudio in vitro de la acción acaricida del aceite esencial de la gramínea Citronela de Java (Cymbopogon winterianus Jowitt) en la garrapata Boophilus microplus. Rev Bras Pl Med 8(2):71–78

    CAS  Google Scholar 

  • Martins RM, González FHD (2007) Uso del aceite de citronela de Java (Cymbopogon winterianus Jowitt) (Panicoidideae) como acaricida frente a la garrapata Boophilus microplus Canestrini (Acari:Ixodidae). Rev Bras Pl Med 9(4):1–8

    CAS  Google Scholar 

  • Massard CL, Fonseca AH (2004) Carrapatos e doenças transmitidas, comuns ao homem e aos animais. A Hora Veterinária 135:15–23

    Google Scholar 

  • Mazzafera P (2003) Efeito alelopático do extrato alcoólico do cravo-da-índia e eugenol. Rev Bras Bot 26(2):231–238. doi:10.1590/s0100-84042003000200011

    Article  Google Scholar 

  • Monteiro CM, Maturano R, Daemon E, Catunda-Jr FEA, Calmon F, Senra TS, Faza A, Carvalho MG (2012) Acaricidal activity of eugenol on Rhipicephalus microplus (Acari: Ixodidae) and Dermacentor nitens (Acari: Ixodidae) larvae. Parasitol Res 111:1295–1300. doi:10.1007/s00436-012-2964-0

    Article  PubMed  Google Scholar 

  • Nerio LS, Oliveiro-Verbel J, Stashenko E (2010) Repellent activity of essential oils: a review. Bioresour Technol 101:372–378. doi:10.1016/j.biortech.2009.07.048

    Article  CAS  PubMed  Google Scholar 

  • Oliveira RA, Reis TV, Sacramento CK, Duarte LP, Oliveira FFO (2009) Constituintes químicos voláteis de especiarias ricas em eugenol. Rev Bras Farmacogn 19(3):771–775. doi:10.1590/S0102-695X2009000500020

    Article  Google Scholar 

  • Olivo CJ, Carvalho NM, Silva JHS, Vogel FF, Massariol P, Meinerz G, Agnolin CA, Morel AF, Viau LV (2008) Óleo de citronela no controle do carrapato de bovinos. Ciênc Rural 38(2):406–410. doi:10.1590/S0103-84782008000200018

    Article  CAS  Google Scholar 

  • Raynal JT, Silva AAB, Souza TJ, Bahiense TC, Meyer R, Portela RW (2013) Acaricides efficiency on Rhipicephalus (Boophilus) microplus from Bahia state North-Central region. Rev Bras Parasitol Vet 22(1):71–77. doi:10.1590/S1984-29612013005000006

    Article  PubMed  Google Scholar 

  • Rodríguez–Rojo S, Varona S, Núñez M, Cocero MJ (2012) Characterization of rosemary essential oil for biodegradable emulsions. Ind Crop Prod 37:137–140. doi:10.1016/j.indcrop.2011.11.026

    Article  Google Scholar 

  • Santos AV, Oliveira RA, Albuquerque GR (2012a) Efeito in vitro do extrato de Nim (Azadirachta indica) e oleo essencial de cravo (Syzygium aromaticum) sobre Rhipicephalus (Boophilus) microplus. Rev Bras Med Vet 34(2):111–115

    Google Scholar 

  • Santos FCC, Vogel FSF, Monteiro SG (2012b) Efeito de diferentes concentrações de óleo e tintura de citronela sobre os parâmetros reprodutivos de teleóginas de Rhipicephalus (Boophilus) microplus. Semin-Ciênc Agrar 33(3):1141–1148

    Article  Google Scholar 

  • Wany A, Jha S, Nigam VK, Pandey DM (2013) Chemical analysis and therapeutic uses of citronella oil from Cymbopogon winterianus: a short review. Int J Adv Res 1(6):504–521

    CAS  Google Scholar 

  • Zeringóta V, Senra TOS, Calmon F, Maturano R, Faza AP, Catunda-Junior FEA, Monteiro CMO, Carvalho MG, Daemon E (2013) Repellent activity of eugenol on larvae of Rhipicephalus microplus and Dermacentor nitens (Acari: Ixodidae). Parasitol Res. doi:10.1007/s00436-013-3434-z

    Google Scholar 

Download references

Acknowledgments

The authors are grateful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil) for the financial support provided by the scholarship.

Conflict of interest

The authors declare no conflicts of interest.

Author information

Authors and Affiliations

  1. Departamento Farmacêutico, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Campus Universitário, 36036-900, Juiz de Fora, Minas Gerais, Brazil

    Valéria de Mello, Luciane Santos da Silva, Flávia del Gaudio Guimarães, Fernanda Maria Pinto Vilela & Maria da Penha Henriques do Amaral

  2. Embrapa Gado de Leite, Juiz de Fora, Minas Gerais, Brazil

    Márcia Cristina de Azevedo Prata & Márcio Roberto da Silva

  3. Departamento de Comportamento e Biologia Animal, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Campus Universitário, Juiz de Fora, Minas Gerais, Brazil

    Erik Daemon

  4. Departamento de Biologia Celular e Molecular, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil

    Alessandra Ésther de Mendonça & Evelize Folly

  5. Laboratório de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Lilian Henriques do Amaral & Lucio Mendes Cabral

Authors
  1. Valéria de Mello
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Márcia Cristina de Azevedo Prata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Márcio Roberto da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Erik Daemon
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luciane Santos da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Flávia del Gaudio Guimarães
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Alessandra Ésther de Mendonça
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Evelize Folly
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Fernanda Maria Pinto Vilela
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Lilian Henriques do Amaral
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Lucio Mendes Cabral
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Maria da Penha Henriques do Amaral
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Maria da Penha Henriques do Amaral.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

de Mello, V., Prata, M.C.d.A., da Silva, M.R. et al. Acaricidal properties of the formulations based on essential oils from Cymbopogon winterianus and Syzygium aromaticum plants. Parasitol Res 113, 4431–4437 (2014). https://doi.org/10.1007/s00436-014-4121-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-014-4121-4

Keywords

Search

Navigation