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Could the homologous sequence of anti-inflammatory pentapeptide (MLIF) produced by Entamoeba histolytica in the N protein of rabies virus affect the inflammatory process?

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Abstract

Amebiasis and rabies are public health problems, and they have in common a poor inflammatory effect in the target organs that they affect. In the GenBank, it was found that the anti-inflammatory peptide monocyte locomotion inhibitory factor (MLIF) produced by Entamoeba histolytica homologates 80%, with a fragment of the N protein of the rabies virus. We speculated if the N protein could contribute to the scant inflammatory reaction produced by rabies virus in central nervous system. The N protein was obtained and studied in vitro and in vivo. The N protein, as MLIF, inhibited the respiratory burst in human mononuclear phagocytes (43%, p<0.05), but in contrast to MLIF, it increased chemotaxis and it did not significantly inhibit delayed hypersensitivity skin reaction to 1-chloro-2-4-dinitrobenzene in guinea pigs. Therefore, the full peptide sequence has to be present or it has to be cleaved—free from the large recombinant N protein molecule (55 kDa) to become active.

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Abbreviations

BACNF:

forward primer

BACNR:

reverse primer

CNS:

central nervous system

DNCB:

1-chloro-2-4-dinitrobenzene

FBS:

fetal bovine serum

IC:

intracerebral

LB:

Luria broth

IMSS:

Instituto Mexicano del Seguro Social

MLIF:

monocyte locomotion inhibitory factor

MP:

mononuclear phagocyte/monocyte

OZ:

opsonizated zymosan

PBS:

phosphate-buffered saline solution

Sf:

Spodoptera frugiperda

UAM:

Universidad Autónoma Metropolitana

UNAM:

Universidad Nacional Autónoma de México

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Acknowledgements

In memoriam: our chief and professor Dr. Roberto Kretschmer Schmid, dead March 22, 2005. Thanks to Lic. Alfonso Magallón P. for the excellent artwork and Lic. Laura Nava of Vinculación Académica-Coplada de UAM-Xochimilco for her help. This work was supported by grant from the Consejo Nacional de Ciencia y Tecnología (CONACYT) No. 34214B.

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Authors and Affiliations

  1. Unidad de Investigación Médica en Inmunología, Unidad Médica de Alta Especialidad, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Av. Cuauhtemoc, No. 330, Col. Doctores, CP 06720, Mexico City, DF, Mexico

    M. E. Morales, G. Rico, R. Silva, J. A. Giménez, R. Kretschmer & A. Aguilar-Setién

  2. Laboratorio de Biomembranas, Departamento de Ciencias de la Salud, UAM Iztapalapa, Mexico City, Mexico

    J. L. Gómez

  3. Laboratorio de Genética Molecular, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico City, DF, Mexico

    R. Alonso & R. Cortés

Authors
  1. M. E. Morales
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  2. G. Rico
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  3. J. L. Gómez
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  4. R. Alonso
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  5. R. Cortés
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  6. R. Silva
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  7. J. A. Giménez
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  8. R. Kretschmer
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  9. A. Aguilar-Setién
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Corresponding author

Correspondence to M. E. Morales.

Additional information

This paper is the result of the thesis work for obtaining Ph.D. degree in Ciencias Biológicas por la Universidad Autónoma Metropolitana

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Morales, M.E., Rico, G., Gómez, J.L. et al. Could the homologous sequence of anti-inflammatory pentapeptide (MLIF) produced by Entamoeba histolytica in the N protein of rabies virus affect the inflammatory process?. Parasitol Res 98, 232–236 (2006). https://doi.org/10.1007/s00436-005-0027-5

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  • DOI: https://doi.org/10.1007/s00436-005-0027-5

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