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Catalysis of NADH→NADP+ transhydrogenation by adult Hymenolepis diminuta mitochondria

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Abstract

Hymenolepis diminuta mitochondria catalyze nonenergy-linked and energy-linked NADH→NADP+ transhydrogenations, with the latter driven by electron-transport dependent NADH oxidation (electron transport-driven, ETD) or ATP hydrolysis (ATP-driven, ATPD). Using submitochondrial particles, NADH→NADP+ transhydrogenations were characterized further. ETD and ATPD reactions were enhanced by bovine serum albumin (BSA) and were inhibited by N,N′-dicyclohexylcarbodiimide (DCCD), carbonyl cyanide 3-chlorophenylhydrazone (CCCP), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP), and niclosamide. The nonenergy-linked reaction was unaffected by these additives. Except for DCCD inhibition of the ATPD reaction, BSA mitigated inhibitor effects on energy-linked activities. BSA enhanced NADH oxidase (but not ATPase) activity. Although DCCD inhibited NADH oxidase and ATPase, BSA only lessened oxidase inhibition. With protonophores, an increase in NADH oxidase (but not ATPase) activity was suggested. Oxidase inhibition by rotenone was unaffected by BSA. The ATP-hydrolyzed/NADPH-formed for the ATPD reaction was almost unity. A model for H. diminuta energy-linked transhydrogenation is presented.

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Acknowledgements

This work was supported, in part, by a grant-in aid for research from Sigma Xi to J.P.P. and grant AI-15597 from the National Institutes of Health, United States Public Health Service (USPHS) to C.F.F. Animal research was conducted in accordance with the Animal Welfare Act, the Guide for the Care and Use of Laboratory Animals, and the United States Department of Agriculture (USDA) and USPHS policy at a USDA-registered USPHS assurance holding facility.

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  1. Department of Biological Sciences, Bowling Green State University, Bowling Green, OH, 43403-0212, USA

    J. P. Park & C. F. Fioravanti

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  1. J. P. Park
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  2. C. F. Fioravanti
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Correspondence to C. F. Fioravanti.

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Park, J.P., Fioravanti, C.F. Catalysis of NADH→NADP+ transhydrogenation by adult Hymenolepis diminuta mitochondria. Parasitol Res 98, 200–206 (2006). https://doi.org/10.1007/s00436-005-0020-z

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  • DOI: https://doi.org/10.1007/s00436-005-0020-z

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