Naringin, a natural dietary compound, prevents intestinal tumorigenesis in Apc Min/+ mouse model
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Naringin is a natural dietary flavonoid compound. We aimed to evaluate the effects of naringin on intestinal tumorigenesis in the adenomatous polyposis coli multiple intestinal neoplasia (Apc Min/+) mouse model.
Apc Min/+ mice were given either naringin (150 mg/kg) or vehicle by p.o. gavage daily for 12 consecutive weeks. Mice were killed with ether, and blood samples were collected to assess the concentrations of IL-6 and PGE2. Total intestines were removed, and the number of polyps was examined. Tissue samples of intestinal polyps were subjected to the assays of histopathology, immunohistochemical analysis and Western blotting analysis.
Apc Min/+ mice fed with naringin developed less and smaller polyps in total intestines. Naringin prevented intestinal tumorigenesis without adverse effects. Histopathologic analysis revealed the reduction of dysplastic cells and dysplasia in the adenomatous polyps. The treatments’ effects might arise from its anti-proliferation, induction of apoptosis and modulation of GSK-3β and APC/β-catenin signaling pathways. Naringin also exerted its effects on tumorigenesis through anti-chronic inflammation.
Naringin prevented intestinal tumorigenesis likely through a collection of activities including anti-proliferation, induction of apoptosis, modulation of GSK-3β and APC/β-catenin pathways and anti-inflammation. Naringin is a potential chemopreventive agent for reducing the risk of colonic cancers.
KeywordsNaringin ApcMin/+ mouse model Intestinal adenomatous polyps APC/β-catenin signaling pathway Anti-proliferation Apoptosis Anti-inflammation
This project was supported by the Natural Science Foundation of China (91229113, 81173090, 81373435) and the Natural Science Foundation of Shandong (ZR2009CQ019).
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Conflict of interest
We confirm that there is no potential conflict of interest or financial dependence regarding this paper.
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