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Identification of potential serum markers for endometrial cancer using protein expression profiling

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Abstract

Objectives

Screening method of endometrial cancer (EC) has not been established yet. Our study was to explore serum biomarkers of EC patients using surface-enhanced laser desorption and ionization-time-of-flight mass spectrometry (SELDI-TOF MS).

Methods

Serum samples from 65 EC patients and 40 controls were analyzed by SELDI-TOF MS (training set). Single- and multi-variant analyses were performed to compare protein profiles in serum of EC patients and healthy controls. Subsequently, blind test set including 40 EC patients and 40 controls were analyzed for validation.

Results

A panel of four biomarker candidates were selected in training set analysis. These markers could also distinguish stage I patients from controls. Among them, two biomarkers were purified and identified as apolipoprotein A1 and a modified form of apolipoprotein C1. Screening for blind test set using dual-biomarker analysis yielded a sensitivity of 82% and a specificity of 86%.

Conclusions

Involvement of apolipoproteins with EC is first suggested in this study. In addition to possibility of screening method for EC, findings of these new biomarkers might be related with carcinogenesis or predisposition to EC.

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Conflict of interest statement

We declare that we have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, National Defense Medical College, Tokorozawa, Saitama, 359-8513, Japan

    Masashi Takano & Tomoko Goto

  2. Department of Gynecology, Ohki Memorial Kikuchi Cancer Clinic for Women, Tokorozawa, Saitama, 359-1133, Japan

    Yoshihiro Kikuchi & Takayoshi Asakawa

  3. Department of Obstetrics and Gynecology, Teikyo Medical University, Itabashi-ku, Tokyo, 8606, Japan

    Tsunekazu Kita

  4. Department of Obstetrics and Gynecology, National Hospital Organization Nishi Saitama Chuo Hospital, Tokorozawa, Saitama, 359-1151, Japan

    Kazuya Kudoh

  5. Cancer Center, Tottori University Hospital, Yonago, Tottori, 683-8505, Japan

    Junzo Kigawa

  6. Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Hokkaido, 060-8648, Japan

    Noriaki Sakuragi

  7. Department of Gynecology, Sasaki Research Institute Kyoundo Hospital, Chiyoda-ku, Tokyo, 101-0062, Japan

    Masaru Sakamoto

  8. Department of Obstetrics and Gynecology, Iwate Medical University, Morioka, Iwate, 020-8505, Japan

    Toru Sugiyama

  9. Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Miyagi, 980-8574, Japan

    Nobuo Yaegashi

  10. Department of Obstetrics and Gynecology, Keio University School of Medicine, Shinano-machi, Shinjuku, Tokyo, 160-8582, Japan

    Hiroshi Tsuda

  11. Department of Obstetrics and Gynecology, Seto Hospital, Tokorozawa, Saitama, 359-1128, Japan

    Hiroshi Seto

  12. Life Science Division, Bio-Rad Laboratories K.K., Yokohama, Kanagawa, 240-0005, Japan

    Mieko Shiwa

Authors
  1. Masashi Takano
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  2. Yoshihiro Kikuchi
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  3. Takayoshi Asakawa
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  4. Tomoko Goto
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  5. Tsunekazu Kita
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  6. Kazuya Kudoh
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  7. Junzo Kigawa
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  8. Noriaki Sakuragi
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  9. Masaru Sakamoto
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  10. Toru Sugiyama
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  11. Nobuo Yaegashi
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  12. Hiroshi Tsuda
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  13. Hiroshi Seto
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  14. Mieko Shiwa
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Corresponding author

Correspondence to Yoshihiro Kikuchi.

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Takano, M., Kikuchi, Y., Asakawa, T. et al. Identification of potential serum markers for endometrial cancer using protein expression profiling. J Cancer Res Clin Oncol 136, 475–481 (2010). https://doi.org/10.1007/s00432-009-0680-7

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  • DOI: https://doi.org/10.1007/s00432-009-0680-7

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