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Prevalence and genetic diversity of norovirus genogroup II in children less than 5 years of age with acute gastroenteritis in Tehran, Iran

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Abstract

Viral gastroenteritis is a major public health problem worldwide. In Iran, very limited studies have been performed with regard to the epidemiology of noroviruses. This study aimed to evaluate the prevalence and molecular epidemiology of GII noroviruses in hospitalized children less than 5 years of age with acute gastroenteritis (AGE). A total of 210 stool specimens were collected from Ali Asghar Children’s Hospital and Bahrami Children’s Hospital in Tehran, from June 2015 to June 2016. The samples were screened by real-time RT-PCR for genogroup II (GII). Positive samples were genotyped by semi-nested PCR followed by Sanger sequencing and phylogenetic analysis. Norovirus was identified in 36 (17.1%) of 210 specimens. Based on genetic analysis of RdRp and capsid sequences, the strains were clustered into eight RdRp–capsid genotypes: GII.P4–GII.4 Sydney_2012 (41.7%), GII.Pe–GII.4 Sydney_2012 (30.6%), GII.P21–GII.3 (13.9%), GII.P16–GII.4 Sydney_2012 (2.8%), GII.P16–GII.12 (2.8%), GII.P2–GII.4 Sydney_2012 (2.8%), GII.P7–GII.7 (2.8%) and GII.P2–GII.2 (2.8%). We determined several different co-circulating norovirus genotypes in children < 5 years of age with AGE in our hospital in Tehran, Iran. Continued molecular surveillance of noroviruses, including typing of both RdRp and capsid genes, is important for monitoring emerging strains in our continued efforts to reduce the overall burden of norovirus disease.

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Acknowledgements

We thank Ali Asghar and Bahrami Children’s Hospitals in Tehran for providing clinical samples.

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Authors and Affiliations

  1. Biology Department, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran

    Mahsa Farsi, Fatemeh Roodbari & Behrooz Nejati

  2. Virology Department, Pasteur Institute of Iran, Tehran, Iran

    Arash Arashkia & Zabihollah Shoja

  3. Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

    Somayeh Jalilvand & Sayed Mahdi Marashi

  4. Ali-Asghar Children’s Hospital, Iran University of Medical Sciences, Tehran, Iran

    Alireza Nateghian

  5. Department of Pediatric Infectious Diseases, Bahrami Children Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Aliakbar Rahbarimanesh

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  1. Mahsa Farsi
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Farsi, M., Roodbari, F., Nejati, B. et al. Prevalence and genetic diversity of norovirus genogroup II in children less than 5 years of age with acute gastroenteritis in Tehran, Iran. Med Microbiol Immunol 207, 201–210 (2018). https://doi.org/10.1007/s00430-018-0541-6

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