Abstract
The fastigial nucleus (FN) is a bilateral cerebellar integrative center for saccadic and vestibular control associated with non-motor functions such as feeding and cardiovascular regulation. In a previous study, we identified a tract of myelinated axons embedded in the subventricular zone (SVZ) that is located between the ependymal cells that form the dorsal wall of the ventricle and the glia limitans at the roof of the fourth ventricle González-González (Sci Rep 2017, 7:40768). Here, we show that this tract of axons, named subventricular axons or SVa, contains projection neurons that bilaterally interconnect both FNs. The approach consisted of the use of a battery of fluorescent neuronal tracers, transgenic mouse lines, and immunohistofluorescence. Our observations show that the SVa belong to a wide network of GABAergic projection neurons mainly located in the medial and caudal region of the FN. The SVa should be considered a part of a continuum of the cerebellar white matter that follows an alternative pathway through the SVZ, a region closely associated with the physiology of the fourth ventricle. This finding adds to our understanding of the complex organization of the FN; however, the function of the interconnection remains to be elucidated.
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Abbreviations
- BG:
-
Bergmann glia
- Cb:
-
Cerebellum
- CFS:
-
Cerebrospinal fluid
- DC:
-
Dorsal cochlear nu
- ECs:
-
Ependymal cells
- ECL:
-
Ependymal cell layer
- FG:
-
Fluro-gold
- Fl:
-
Flocculus
- FN:
-
Fastigial nucleus
- FR:
-
Fluoro-ruby
- GiA:
-
Gigantocellular nu
- icp:
-
Inferior cerebellar peduncle
- IntA:
-
Anterior interpositus
- LVe:
-
Lateral vestibular nu
- LPGi:
-
Lateral paragigantocellular nu
- Med:
-
Medial (fastigial) nu
- MVePc:
-
Medial vestibular nu (parvocellular)
- MVeMC:
-
Medial vestibular nu (magnocellular)
- nu:
-
Nucleus
- Pc:
-
Purkinje cell
- pcn:
-
Precentral fissure, cerebellum
- PnC:
-
Pontine reticular nu
- Pr:
-
Prepositus hypoglossal nu
- py:
-
Pyramidal tract
- P7:
-
Prefacial zone
- SpVe:
-
Spinal vestibular nu
- Sp5:
-
Spinal trigeminal tract
- SuVe:
-
Superior vestibular nu
- SVa:
-
Subventricular axons
- SVCC:
-
Subventricular cellular cluster
- Unc:
-
Uncinate fasciculus
- VCP:
-
Ventral cochlear posterior nu
- VeCb:
-
Vestibulocerebellar nu
- VMC:
-
Ventromedial cord
- X:
-
Cerebellar lobule ten
- x:
-
X nu
- I:
-
Cerebellar lobule one
- 4 V:
-
Fourth ventricle
- 7 N:
-
Facial nu
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Acknowledgements
This project was supported by grants from Consejo Nacional de Ciencia y Tecnología (CONACyT) (A1S7659) and Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT-DGAPA) (IN204520) to AMT. GBGG is a doctoral student from Programa de Doctorado en Ciencias Biomédicas, at Universidad Nacional Autónoma de México (UNAM), and was supported by CONACyT fellowship 277694. For the kindly donation of mice and materials, we thank Dr. Annalisa Buffo (NICO Institute-Italy), M. Condés-Lara and G. Rojas Piloni (Instituto de Neurobiología-INB, UNAM, Mexico), and H. Kettenmann (MDC-Berlin). We thank Dr. D. Escalante (IFC-UNAM) and R. Arellano (INB-UNAM) for suggestions and feedback. We thank the INB-Core Facilities for their technical support: unit of Microscopy (L. Palma-Tirado, E. N. Hernández-Ríos), and animal facility (A. Castilla-Leon, M. García-Servin, M.A. Carbajo). We also appreciate the technical backing of G. Martinez-Lorenzana, R. Olivares-Moreno and A.E. Espino Saldaña; the input and manuscript editing by M.A. González-González; and the English grammar editing by Dr. M. C. Jeziorski and J. G. Norris.
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Funding provided to Consejo Nacional de Ciencia y Tecnología (MX): 277694; Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México: IN204520; Consejo Nacional de Ciencia y Tecnología (MX): A1S7659.
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GBGG conducted the experiments. AMT and GBGG designed the work, analyzed data, and wrote the paper.
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Gómez-González, G.B., Martínez-Torres, A. Inter-fastigial projections along the roof of the fourth ventricle. Brain Struct Funct 226, 901–917 (2021). https://doi.org/10.1007/s00429-021-02217-8
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DOI: https://doi.org/10.1007/s00429-021-02217-8