Prognostic significance of 1p36 locus deletion in adenoid cystic carcinoma of the salivary glands

  • Petr Šteiner
  • Simon Andreasen
  • Petr Grossmann
  • Lukáš Hauer
  • Tomáš Vaněček
  • Markéta Miesbauerová
  • Thalita Santana
  • Katalin Kiss
  • David Slouka
  • Alena Skálová
Original Article


Adenoid cystic carcinoma (AdCC) of the salivary glands is characterized by MYB-NFIB or MYBL1-NFIB fusion, prolonged but relentlessly progressive clinical course with frequent recurrences, and development of distant metastasis resulting in high long-term mortality. Currently, no effective therapy is available for patients with advanced non-resectable and/or metastatic disease. Complicating the clinical management of this patient group is the lack of prognostic markers. The purpose of this study is to investigate the prognostic value of 1p36 loss in patients with AdCC. The presence of 1p36 deletion and gene fusions involving the MYB, NFIB, and MYBL1 genes in a cohort of 93 salivary gland AdCCs was studied using fluorescence in situ hybridization. These results were statistically correlated with clinical data and outcome. Deletion of 1p36 in AdCC was identified in 13 of 85 analyzable cases (15.29%). MYB-NFIB fusion was detected in 57/85 (67.1%), MYBL1-NFIB fusion in 12/85 (14.1%), MYB-X fusion in 4/85 (4.7%), MYBL1-X in 4/85 (4.7%), and NFIB-X in 2/85 (2.4%) of AdCC cases. None of the 1p36-deleted samples showed MYBL1 rearrangement. Statistical analysis demonstrated a significant correlation between 1p36 deletion and advanced tumor stage and solid histology (p = 0.0061 and 0.0007, respectively). Kaplan-Meier survival curves showed statistically significant correlations between 1p36 deletion and decreased overall survival, disease-specific survival, recurrence-free interval, and recurrence-free survival, all of which were maintained in multivariate analysis. We demonstrate that 1p36 deletion can serve as an indicator of unfavorable outcome of patients with salivary gland AdCC.


Salivary gland Adenoid cystic carcinoma 1p36 locus deletion MYB-NFIB Prognosis 



We wish to thank Stanislav Kormunda for valuable assistance with statistical analysis.


This study was supported by the Ministry of Education, Czech Republic (grant SVV-2017-260 391).

Compliance with ethical standards

Study design has been approved by Danish Regional Ethics Committee (H-6-2014-086), the Danish Data Protection Agency (REG-94-2014), and local ethical committee of Charles University, Faculty of Medicine Plzen.

Conflicts of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Petr Šteiner
    • 1
    • 2
  • Simon Andreasen
    • 3
    • 4
  • Petr Grossmann
    • 2
  • Lukáš Hauer
    • 5
  • Tomáš Vaněček
    • 1
    • 2
  • Markéta Miesbauerová
    • 1
  • Thalita Santana
    • 6
  • Katalin Kiss
    • 7
  • David Slouka
    • 8
  • Alena Skálová
    • 1
  1. 1.Department of Pathology, Faculty of Medicine in PlzenCharles UniversityPlzenCzech Republic
  2. 2.Bioptic Laboratory, Ltd, Molecular Pathology LaboratoryPlzenCzech Republic
  3. 3.Department of Otorhinolaryngology Head and Neck Surgery and Audiology, RigshospitaletCopenhagen University HospitalCopenhagenDenmark
  4. 4.Department of Otorhinolaryngology and Maxillofacial SurgeryZealand University HospitalKøgeDenmark
  5. 5.Department of Maxillofacial Surgery, Faculty of Medicine in Plzen, Clinic of DentistryCharles UniversityPlzenCzech Republic
  6. 6.Department of Oral Pathology, Faculty of DentistryUniversity of São PauloSão PauloBrazil
  7. 7.Department of Pathology, RigshospitaletCopenhagen University HospitalCopenhagenDenmark
  8. 8.Department of Otorhinolaryngology, Faculty of Medicine in PlzenCharles UniversityPlzenCzech Republic

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