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Virchows Archiv

, Volume 471, Issue 1, pp 23–30 | Cite as

High neuronatin (NNAT) expression is associated with poor outcome in breast cancer

  • Norbert Nass
  • Sarah Walter
  • Dörthe Jechorek
  • Christine Weissenborn
  • Atanas Ignatov
  • Johannes Haybaeck
  • Saadettin Sel
  • Thomas Kalinski
Original Article

Abstract

Neuronatin (NNAT) is a proteolipid involved in cation homeostasis especially in the developing brain. Its expression has been associated with the progression of lung cancer, glioblastoma, and neuroblastoma as well as glucose induced apoptosis in pancreatic cells. We performed a retrospective study of 148 breast cancer specimens for NNAT expression by immunohistochemistry to evaluate this protein as a prognostic marker for breast cancer. We found a high NNAT immunoreactivity score (by multivariate cox regression) to be an independent prognostic marker for relapse-free (hazard ratio HR = 3.55, p = 0.002) and overall survival (HR = 6.29, p < 0.001). However, NNAT expression was not associated with classical parameters such as hormone receptor expression (p = 0.86) or lymph node metastasis (p = 0.83). Additional independent risk factors in this study population were tumor size (≤2 cm; overall survival: HR = 0.36, p = 0.023; relapse-free survival: HR = 0.26, p < 0.01) and blood vessel infiltration (overall survival: HR = 0.34 p < 0.01). NNAT expression determined by immunohistochemistry might therefore become a helpful additional biomarker to identify high-risk breast cancer patients.

Keywords

Breast cancer Neuronatin Immunohistochemistry Prognosis 

Notes

Acknowledgments

Parts of this study have been supported by a grant of the Deutsche Forschungsgemeinschaft (DFG) to T.K. (KA2663/3-1). Professional assistance of our immunohistological laboratory, especially Carola Kügler, Claudia Miethke, and Nadine Wiest, is greatly appreciated.

Compliance with ethical standards

Funding

This study was funded by a grant of the Deutsche Forschungsgemeinschaft (DFG) to T.K. (KA2663/3-1).

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Department of PathologyOtto von Guericke University MagdeburgMagdeburgGermany
  2. 2.Department of Obstetrics and GynecologyOtto von Guericke University MagdeburgMagdeburgGermany
  3. 3.Department of OphthalmologyUniversity of HeidelbergHeidelbergGermany

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