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Fine mapping of powdery mildew resistance gene Pm4e in bread wheat (Triticum aestivum L.)

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A Correction to this article was published on 05 September 2018

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Abstract

Main conclusion

Fine mapping of wheat powdery mildew-resistance gene Pm4e to a 0.19 cM interval with sequence-based markers provides the foundation for map-based cloning and marker-assisted selection with breeder-friendly markers.

Powdery mildew caused by Blumeria graminis f. sp. tritici is a wheat foliar disease that poses a serious threat to global wheat production. Pm4 is a resistance gene locus that has played a key role in controlling this disease in wheat production and a few resistance alleles of this locus have been identified. We have previously mapped the Pm4e allele to a 6.7 cM interval on chromosome 2AL. In this study, Pm4e was delimited to a 0.19 cM interval flanked by Xwgrc763 and Xwgrc865, through employment of a larger segregating population, derived from the cross of resistant parent D29 with susceptible parent Yangmai 158 (Y158), and enrichment of the genetic interval with markers developed on Chinese Spring (C.S.) survey sequence. In this interval, Pm4e co-segregated with a few markers, some of which were either D29-dominant or Y158-dominant, implying great sequence variation in the interval between D29 and Y158. Most of these co-segregation markers could not differentiate the Pm4 alleles from each other. Survey of 55 wheat cultivars with four co-dominant markers showed that the Pm4e-co-segregating loci always co-exist. Annotation of the Pm4e interval-corresponding C.S. sequence revealed more than a dozen resistance gene analogs clustered in a 2.4 Mb region, although C.S. is susceptible to the Pm4e-avirulent isolate Bgt2. This study has established the foundation for map-based cloning of Pm4e. Moreover, some of the co-dominant markers developed in this study could help in marker-assisted transfer of Pm4e into elite cultivars.

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Change history

  • 05 September 2018

    Unfortunately, the style of the units was incorrectly published (“cm” instead of “cM”) throughout the original article.

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Acknowledgements

This project was partially supported by National Key Research and Development Program (2016YFD0101802), NSFC Funds 30025030, 30771344, 30771165 and 31501302, Fundamental Research Fund for the Central Universities (KJQN201633), Jiangsu Collaborative Innovation Initiative for Modern Crop Production, ‘111’ project B08025, Innovation Team Program for Jiangsu Universities (2014). The first author also acknowledges the financial support from Chinese Scholarship Council for Ph. D program.

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  1. Khan Nasr Ullah and Na Li contributed equally.

Authors and Affiliations

  1. The Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Khan Nasr Ullah, Na Li, Tao Shen, Peisi Wang, Wenbin Tang, Shengwei Ma, Zhimeng Zhang, Haiyan Jia, Zhongxin Kong & Zhengqiang Ma

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  1. Khan Nasr Ullah
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The original version of this article was revised: The incorrect units has been corrected.

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Ullah, K.N., Li, N., Shen, T. et al. Fine mapping of powdery mildew resistance gene Pm4e in bread wheat (Triticum aestivum L.). Planta 248, 1319–1328 (2018). https://doi.org/10.1007/s00425-018-2990-y

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