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Magnesium uptake characteristics in Arabidopsis revealed by 28Mg tracer studies

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Abstract

Main conclusion

The Mg2+ uptake system in Arabidopsis roots is Gd3+- and Fe2+-sensitive, and responds to a changing Mg2+ concentration within 1 h with the participation of AtMRS2 transporters.

Abstract

Magnesium (Mg2+) absorption and the mechanism regulating its activity have not been clarified yet. To address these issues, it is necessary to reveal the characteristics of Mg2+ uptake in roots. Therefore, we first investigated the Mg2+ uptake characteristics in roots of 1-week-old Arabidopsis plants using 28Mg. The Mg2+ uptake system in roots was up-regulated within 1 h in response to the low Mg2+ condition. This induction was inhibited in Arabidopsis “mitochondrial RNA splicing 2/magnesium transport” mutants atmrs2-4/atmgt6 and atmrs2-7/atmgt7, while the expression of AtMRS2-4/AtMGT6 and AtMRS2-7/AtMGT7 genes in the Arabidopsis wild-type was not responsive to Mg2+ conditions. In addition, the Mg deficiency-induced Mg2+ uptake system was shut-down within 5 min when Mg2+ was resupplied to the environment. An inhibition study showed that the constitutive mechanism functioning in Mg2+ uptake under Mg2+ sufficient conditions was sensitive to a number of divalent and trivalent cations, particularly Gd3+ and Fe2+, but not to K+.

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Fig. 1
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Abbreviations

CoHEX:

Hexammine cobalt(III) chloride

MGT:

Magnesium transport

MRS2:

Mitochondrial RNA splicing 2

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Acknowledgements

Authors thank Dr. Martin O’Brien for English editing. This work was partially supported by JSPS KAKENHI Grant-in-Aid for Young Scientists (B) (number 17K15236), JSPS KAKENHI Grant-in-Aid for Scientific Research (A) (15H02469) and JST PROSTO (number JPMJPR15Q7). This work was also sponsored by JSPS and F.R.S.-FNRS under the Japan - Belgium Research Cooperative Programme.

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  1. Takaaki Ogura and Natsuko I. Kobayashi contributed equally.

Authors and Affiliations

  1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Takaaki Ogura, Natsuko I. Kobayashi, Tomoko M. Nakanishi & Keitaro Tanoi

  2. National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba, Chiba, 263-8555, Japan

    Hisashi Suzuki

  3. Cyclotron and Radioisotope Center, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan

    Ren Iwata

  4. PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan

    Keitaro Tanoi

Authors
  1. Takaaki Ogura
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  2. Natsuko I. Kobayashi
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  3. Hisashi Suzuki
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  4. Ren Iwata
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  5. Tomoko M. Nakanishi
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  6. Keitaro Tanoi
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Correspondence to Keitaro Tanoi.

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Ogura, T., Kobayashi, N.I., Suzuki, H. et al. Magnesium uptake characteristics in Arabidopsis revealed by 28Mg tracer studies. Planta 248, 745–750 (2018). https://doi.org/10.1007/s00425-018-2936-4

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