Abstract
Main conclusion
The histone acetyltransferase HAF2 facilitates H3 acetylation deposition at the PRR5 and LUX promoters to contribute to robust circadian oscillation.
The circadian clock ensures synchronization of endogenous rhythmic processes with environmental cycles. Multi-layered regulation underlies precise circadian oscillation, and epigenetic regulation is emerging as a crucial scheme for robust circadian maintenance. Here, we report that HISTONE ACETYLTRANSFERASE OF THE TAFII250 FAMILY 2 (HAF2) is involved in circadian homeostasis. The HAF2 gene is activated at midday, and its temporal expression is shaped by CIRCADIAN CLOCK-ASSOCIATED 1. The midday-activated HAF2 protein stimulates H3 acetylation (H3ac) deposition at the PRR5 and LUX loci, contributing to establishment of the raising phase. These results indicate that epigenetic waves in circadian networks underlie temporal compartmentalization of circadian components and stable maintenance of circadian oscillation.
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Abbreviations
- CCA1:
-
CIRCADIAN CLOCK-ASSOCIATED 1
- ChIP:
-
Chromatin immunoprecipitation
- H3ac:
-
Histone 3 acetylation
- HAF2:
-
HISTONE ACETYLTRANSFERASE OF THE TAFII250 FAMILY 2
- LHY:
-
LATE ELONGATED HYPOCOTYL
- LUX:
-
LUX ARRHYTHMO
- PRR:
-
PSEUDO-RESPONSE REGULATOR
- ZT:
-
Zeitgeber time
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Acknowledgements
This work was supported by the Basic Science Research (NRF-2016R1D1A1B03931139) and Basic Research Laboratory (NRF-2017R1A4A1015620) programs provided by the National Research Foundation of Korea and by the Next-Generation BioGreen 21 Program (PJ01314501) provided by the Rural Development Administration.
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Lee, K., Seo, P.J. The HAF2 protein shapes histone acetylation levels of PRR5 and LUX loci in Arabidopsis. Planta 248, 513–518 (2018). https://doi.org/10.1007/s00425-018-2921-y
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DOI: https://doi.org/10.1007/s00425-018-2921-y