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Planta

, Volume 248, Issue 1, pp 185–196 | Cite as

Nitrate inhibits the remobilization of cell wall phosphorus under phosphorus-starvation conditions in rice (Oryza sativa)

  • Chun Quan Zhu
  • Xiao Fang Zhu
  • Chao Wang
  • Xiao Ying Dong
  • Ren Fang Shen
Original Article
  • 255 Downloads

Abstract

Main conclusion

NO3 not only inhibited the reutilization of cell wall P via decreasing root cell wall pectin content and PME activity, but also hampered the P translocation from root to shoot.

The rice cultivars ‘Kasalath’ (Kas) and ‘Nipponbare’ (Nip) were used to demonstrate that the nitrogen source NO3 inhibits internal phosphorus (P) reutilization in rice under P-absence conditions. Analysis using Kas showed that the expression of − P-induced marker genes OsIPS1/2 and OsSPX1/2/3/5 are significantly higher under 1 mM NO 3 −  − P (1N − P) treatment than 0 mM NO 3 −  − P (0N − P) treatment. The absence of NO3 from the nutrient solution significantly increased cell wall P release by increasing pectin synthesis and increasing the activity of pectin methylesterase (PME), and also significantly improved the translocation of soluble P from the root to the shoot by increasing xylem sap P content under P-absence conditions. The rice seedlings grown in 0 mM NO3 accumulated significantly higher nitric oxide (NO) in the roots than those grown in 1 mM NO3. Exogenously applying the NO donor sodium nitroprusside (SNP) revealed that NO is a major contributor to differential cell wall P remobilization in rice by mediating pectin synthesis and demethylation under different NO3 concentrations (0 and 1 mM) under P-deprived conditions.

Keywords

Cell wall Nitrate (NO3Nitric oxide (NO) Phosphorus (P) Remobilization Rice Translocation 

Abbreviations

NO

Nitric oxide

NR

Nitrate reductase

PME

Pectin methylesterase

SNP

Sodium nitroprusside

Notes

Acknowledgements

This work was funded by the National Key Basic Research Program of China (Grant number 2014CB441000), the Natural Science Foundation of China (Grant number 31501825), and the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant numbers XDB15030302 and XDB15030202).

Supplementary material

425_2018_2892_MOESM1_ESM.docx (56 kb)
Supplemental Fig. S1 The contents of NH4+ and NO3 in Kas and Nip rice cultivars under P-sufficient and P-deficient conditions. Seedlings were grown in P-sufficient or P-deficient nutrient solutions for 1 week. The roots and shoots were collected to measure the NH4+ and NO3 contents. Columns with asterisks are significantly different at P < 0.05. Data are mean ± SD (n = 4) (DOCX 55 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Chun Quan Zhu
    • 1
    • 2
    • 3
  • Xiao Fang Zhu
    • 1
  • Chao Wang
    • 1
  • Xiao Ying Dong
    • 1
  • Ren Fang Shen
    • 1
    • 2
  1. 1.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil ScienceChinese Academy of ScienceNanjingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.National Key Laboratory of Rice BiologyChina National Rice Research InstituteHangzhouChina

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