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Planta

, Volume 247, Issue 5, pp 1149–1161 | Cite as

An auxin-induced β-type endo-1,4-β-glucanase in poplar is involved in cell expansion and lateral root formation

  • Liangliang Yu
  • Qiong Li
  • Yingying Zhu
  • Muhammad Saddique Afzal
  • Laigeng Li
Original Article

Abstract

Main conclusion

PtrGH9A7, a poplar β-type endo-1,4-β-glucanase gene induced by auxin, promotes both plant growth and lateral root development by enhancing cell expansion.

Endo-1,4-β-glucanase (EGase) family genes function in multiple aspects of plant growth and development. Our previous study found that PtrCel9A6, a poplar EGase gene of the β subfamily, is specifically expressed in xylem tissue and is involved in the cellulose biosynthesis required for secondary cell wall formation (Yu et al. in Mol Plant 6:1904–1917, 2013). To further explore the functions and regulatory mechanism of β-subfamily EGases, we cloned and characterized another poplar β-type EGase gene PtrGH9A7, a close homolog of PtrCel9A6. In contrast to PtrCel9A6, PtrGH9A7 is predominantly expressed in parenchyma tissues of the above-ground part; in roots, PtrGH9A7 expression is specifically restricted to lateral root primordia at all stages from initiation to emergence and is strongly induced by auxin application. Heterologous overexpression of PtrGH9A7 promotes plant growth by enhancing cell expansion, suggesting a conserved role for β-type EGases in 1,4-β-glucan chains remodeling, which is required for cell wall loosening. Moreover, the overexpression of PtrGH9A7 significantly increases lateral root number, which might result from improved lateral root primordium development due to enhanced cell expansion. Taken together, these results demonstrate that this β-type EGase induced by auxin signaling has a novel role in promoting lateral root formation as well as in enhancing plant growth.

Keywords

Endo-1,4-β-glucanase Cell expansion Lateral root Lateral root primordium Auxin Poplar 

Abbreviations

EGase

Endo-1,4-β-glucanase

GUS

β-Glucuronidase

LR

Lateral root

LRP

Lateral root primordium

Notes

Acknowledgements

We thank Ms. Jiqin Li at Shanghai Institute of Plant Physiology and Ecology for assistance with scanning electron microscopy. This work was supported by the National Natural Science Foundation of China (31500197, 31630014) and Shanghai Sailing Program (15YF1403800).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

425_2018_2851_MOESM1_ESM.jpg (2.6 mb)
Supplementary material 1 (JPEG 2616 kb) Fig. S1 PtrGH9A7p:GUS is not induced by phytohormones except auxin. a-f Histochemical analyses of PtrGH9A7p:GUS seedlings treated with mock (a), 10 μM 2,4-D (b), GA3 (c), 6-BA (d), ABA (e), ACC (f) for 24 h. Bars = 2 mm
425_2018_2851_MOESM2_ESM.jpg (2.9 mb)
Supplementary material 2 (JPEG 3000 kb) Fig. S2 Representative images of LRPs at developmental stages II to emergence (a to g, respectively) and LRs (h and i) of 11-d-old wild-type and PtrGH9A7-OX transgenic seedlings

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Shanghai Key Lab of Bio-energy Crops, School of Life SciencesShanghai UniversityShanghaiChina
  2. 2.National Key Laboratory of Plant Molecular Genetics and CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghaiChina

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