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Planta

, Volume 247, Issue 5, pp 1109–1122 | Cite as

Differential proteomic analysis reveals sequential heat stress-responsive regulatory network in radish (Raphanus sativus L.) taproot

  • Ronghua Wang
  • Yi Mei
  • Liang Xu
  • Xianwen Zhu
  • Yan Wang
  • Jun Guo
  • Liwang Liu
Original Article

Abstract

Main conclusion

Differential abundance protein species (DAPS) involved in reducing damage and enhancing thermotolerance in radish were firstly identified. Proteomic analysis and omics association analysis revealed a HS-responsive regulatory network in radish.

Heat stress (HS) is a major destructive factor influencing radish production and supply in summer, for radish is a cool season vegetable crop being susceptible to high temperature. In this study, the proteome changes of radish taproots under 40 °C treatment at 0 h (Control), 12 h (Heat12) and 24 h (Heat24) were analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification) approach. In total, 2258 DAPS representing 1542 differentially accumulated uniprotein species which respond to HS were identified. A total of 604, 910 and 744 DAPS was detected in comparison of Control vs. Heat12, Control vs. Heat24, and Heat12 vs. Heat24, respectively. Gene ontology and pathway analysis showed that annexin, ubiquitin-conjugating enzyme, ATP synthase, heat shock protein (HSP) and other stress-related proteins were predominately enriched in signal transduction, stress and defense pathways, photosynthesis and energy metabolic pathways, working cooperatively to reduce stress-induced damage in radish. Based on iTRAQ combined with the transcriptomics analysis, a schematic model of a sequential HS-responsive regulatory network was proposed. The initial sensing of HS occurred at the plasma membrane, and then key components of stress signal transduction triggered heat-responsive genes in the plant protective metabolism to re-establish homeostasis and enhance thermotolerance. These results provide new insights into characteristics of HS-responsive DAPS and facilitate dissecting the molecular mechanisms underlying heat tolerance in radish and other root crops.

Keywords

Differential abundance protein species (DAPS) Heat stress iTRAQ Thermotolerance 

Abbreviations

DAPS

Differential abundance protein species

DEG

Differentially expressed gene

GO

Gene ontology

HS

Heat stress

HSP

Heat shock protein

iTRAQ

Isobaric tag for relative and absolute quantification

Notes

Acknowledgments

This work was in part supported by grants from the National Key Technology Research and Development Program of China (2017YFD0101803; 2017YFD0101806), Jiangsu Agricultural Science and Technology Innovation Fund [JASTIF, CX(16)1012] and the Key Technology Research and Development Program of Jiangsu Province (BE2016379).

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Ronghua Wang
    • 1
    • 2
  • Yi Mei
    • 3
  • Liang Xu
    • 1
  • Xianwen Zhu
    • 4
  • Yan Wang
    • 1
  • Jun Guo
    • 3
  • Liwang Liu
    • 1
  1. 1.National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of HorticultureNanjing Agricultural UniversityNanjingPeople’s Republic of China
  2. 2.Institute of Vegetables and Flowers, Shandong Academy of Agricultural SciencesJinanPeople’s Republic of China
  3. 3.Yancheng Academy of Agricultural SciencesYanchengPeople’s Republic of China
  4. 4.Department of Plant SciencesNorth Dakota State UniversityFargoUSA

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