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Identification of OsMYC2-regulated senescence-associated genes in rice

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Abstract

Main conclusion

The jasmonic acid (JA)-responsive transcription factor OsMYC2 acts as a positive regulator of leaf senescence by direct regulation of some senescence-associated genes in rice.

OsMYC2, a transcription factor (TF), acts as a positive regulator of jasmonic acid (JA) signaling involved in development and defense in rice. Here, we report that OsMYC2 plays an important role in leaf senescence under dark-induced senescence (DIS) conditions. Overexpression of OsMYC2 significantly promoted leaf senescence, indicated by reduction of chlorophyll content under DIS conditions in rice. Leaf senescence under the DIS conditions was negatively regulated by OsJAZ8, a rice jasmonate ZIM-domain protein involved in the JA signaling pathway. OsMYC2 upregulated the expression of some senescence-associated genes (SAGs) and selectively bound to the G-box/G-box-like motifs in the promoters of some SAGs in vivo. These results suggest that OsMYC2 acts as a positive regulator of leaf senescence by direct- or indirect-regulation of SAGs in rice.

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Fig. 1
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Abbreviations

DIS:

Dark-induced senescence

JA:

Jasmonic acid

JAZ:

Jasmonate ZIM domain

PR:

Pathogenesis-related

SAG:

Senescence-associated gene

TF:

Transcription factor

Xoo :

Xanthomonas oryzae pv. oryzae

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Acknowledgements

We thank Drs. Y. Nishizawa (National Institute of Agrobiological Sciences, NIAS) for providing the binary vector. We also thank M. Satoh (National Agricultural Research Center for Kyushu Okinawa Region, NARO) and Dr. H. Kanno (NARO) for laying the foundation of a part of this study. This work was supported by the Japan Society for Promotion of Science (JSPS) [Funding Program for Next Generation World-Leading Researchers (No. GS022)], Kagawa University Young Scientists 2014, and JSPS Research Fellow (No. 16J01564).

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Authors and Affiliations

  1. Plant Genome and Resource Research Center, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795, Japan

    Yuya Uji, Kazuya Akimitsu & Kenji Gomi

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  1. Yuya Uji
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  2. Kazuya Akimitsu
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  3. Kenji Gomi
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Correspondence to Kenji Gomi.

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Uji, Y., Akimitsu, K. & Gomi, K. Identification of OsMYC2-regulated senescence-associated genes in rice. Planta 245, 1241–1246 (2017). https://doi.org/10.1007/s00425-017-2697-5

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