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Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.)

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Abstract

Hybrid sterility hinders the exploitation of the heterosis displayed by japonica × indica rice hybrids. The variation in pollen semi-sterility observed among hybrids between the japonica recipient cultivar and each of two sets of chromosome segment substitution lines involving introgression from an indica cultivar was due to a factor on chromosome 5 known to harbor the gene S24. S24 was fine mapped to a 42 kb segment by analyzing a large F2 population bred from the cross S24-NIL × Asominori, while the semi-sterility shown by the F1 hybrid was ascribable to mitotic failure at the early bicellular pollen stage. Interestingly, two other pollen sterility genes (f5-Du and Sb) map to the same region (Li et al. in Chin Sci Bull 51:675–680, 2006; Wang et al. in Theor Appl Genet 112:382–387, 2006), allowing a search for candidate genes in the 6.4 kb overlap between the three genes. By sequencing the overlapped fragment in wild rice, indica cultivars and japonica cultivars, a protein ankyrin-3 encoded by the ORF2 was identified as the molecular base for S24. A cultivar Dular was found to have a hybrid-sterility-neutral allele, S24-n, in which an insertion of 30 bp was confirmed. Thus, it was possible to add one more case of molecular bases for the hybrid sterility. No gamete abortion is caused on heterozygous maternal genotype with an impaired sequence from the hybrid-sterility-neutral genotype. This result will be useful in understanding of wide compatibility in rice breeding.

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Abbreviations

ANK-3:

Ankyrin-3

CSSLs:

Chromosome segment substitution lines

MMC:

Microspore mother cell

NILs:

Near isogenic lines

ORF:

Open reading frame

SSR:

Simple sequence repeat

WCG:

Wide compatibility gene

WCV:

Wide compatibility variety

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Acknowledgments

We thank Dr. A. Yoshimura (Kyushu University, Japan) for his kind provision of the CSSL populations and associated genotypic data. This research was supported by the Grants from the Chinese National High Technology Research and Development Program (“863” Program, Nos. 2001AA241024; 2003AA222131; 2003AA207020), the Chinese National Natural Science Foundation (30800679) and the “948” project.

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Authors and Affiliations

  1. National Key Laboratory For Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing, 210095, China

    Z. G. Zhao, Y. H. Zhang, X. F. Bian, Y. Wang, L. Jiang, X. Liu, L. M. Chen, S. J. Liu, W. W. Zhang & J. M. Wan

  2. Institute of Crop Science/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), Zhongguancun South Street 12, Beijing, 100081, China

    S. S. Zhu & J. M. Wan

  3. Department of Breeding, Nihon University, 1866 Kameino, Fujisawa, 2528510, Japan

    H. Ikehashi

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  1. Z. G. Zhao
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  4. X. F. Bian
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Corresponding author

Correspondence to J. M. Wan.

Electronic supplementary material

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Supplement Table 1 Nucleotide variation within the S24 sequence in a panel of 14 O. rufipogon accessions and 13 indica cultivars and 11 Japonica cultivars

Supplement Table 2 Primer sequences used to amplify de novo developed molecular markers and re-sequenced are listed

Supplement Fig. 1 Breeding scheme for S24-NIL. CSSL34 includes IR24 segments of chromosomes 4, 5, 6 and 8 in an otherwise Asominori background. The black bars denote segments inherited from IR24

Supplement Fig. 2 Phylogenetic tree of the S24 gene created by the unweighted pair-group method using arithmetic average. Numbers indicate genetic distance in the phylogeny

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Zhao, Z.G., Zhu, S.S., Zhang, Y.H. et al. Molecular analysis of an additional case of hybrid sterility in rice (Oryza sativa L.). Planta 233, 485–494 (2011). https://doi.org/10.1007/s00425-010-1313-8

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