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RLK7, a leucine-rich repeat receptor-like kinase, is required for proper germination speed and tolerance to oxidative stress in Arabidopsis thaliana

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Abstract

The leucine-rich repeat class of receptor-like kinase (LRR-RLKs) encoding genes represents the largest family of putative receptor genes in the Arabidopsis thaliana genome. However, very little is known about the range of biological process that they control. We present in this paper the functional characterization of RLK7 that has all the structural features of a receptor-like kinase of the plant-specific LRR type. To this end, we identified and characterized three independent T-DNA insertion mutants, constructed lines carrying truncated versions of this putative receptor, one lacking the cytoplasmic kinase domain (RLK7Δkin) and the other one lacking 14 LRR repeats (RLK7ΔLRR) and generated RLK7 overexpressing lines. We thus provide evidences that RLK7 is involved in the control of germination speed and the tolerance to oxidant stress. First, consistent with the expression kinetics of the RLK7 gene in the seeds, we found that all three mutants showed a delay in germination, whereas the overexpressors, RLK7Δkin and RLK7ΔLRR lines displayed a phenotype of more precocious germination. Second, a non-hypothesis driven proteomic approach revealed that in the seedlings of the three T-DNA insertion lines, four enzymes directly or indirectly involved in reactive oxygen species detoxification, were significantly less abundant. Consistent with this finding, the three mutants were less tolerant than the wild type to a hydrogen peroxide treatment, whereas the overexpressors, RLK7Δkin and RLK7ΔLRR lines presented the opposite phenotype.

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Abbreviations

LRR:

Leucine-rich repeat

RLK:

Receptor-like kinase

ABA:

Abscisic acid

GA:

Gibberellins

ABI3:

ABSCISIC ACID INSENSITIVE3

FUS3:

FUSCA3

LEC1:

LEAFY COTYLEDON1

ROS:

Reactive oxygen species

GUS:

β-Glucuronidase

2-D DIGE:

Two-dimensional differential in gel electrophoresis

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Acknowledgments

We thank F. Parcy (CNRS, CEA Grenoble, France) for the gift of abi3-6 seeds, M. Devic (CNRS, IRD Montpellier, France) for the gift of lec1-2 seeds and N. Bies-Etheve (UPVD, Perpignan, France) for the abi5-5 mutant. We are grateful to J.P. Brizard (IRD Montpellier, France) for 2-D DIGE analysis and the proteomics platform of Montpellier Genopole for MS analysis. We finally wish to thank two anonymous reviewers for their helpful comments. Financial support was provided by the Centre National de la Recherche Scientifique (CNRS). D.P. was supported by a scholarship from the French Ministry of Research and Education.

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  1. Delphine Pitorre

    Present address: Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9/4, 1030, Vienna, Austria

Authors and Affiliations

  1. Laboratoire Génome et Développement des Plantes, UMR 5096 CNRS-UPVD-IRD, Université de Perpignan, 58 Avenue Paul Alduy, 66860, Perpignan Cedex, France

    Delphine Pitorre, Christel Llauro, Edouard Jobet, Jocelyne Guilleminot, Michel Delseny & Eric Lasserre

  2. Institut de Recherche pour le Développement (IRD), UMR 5096 CNRS-UPVD-IRD, BP 64501, 34394, Montpellier Cedex 5, France

    Jean-Paul Brizard

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  1. Delphine Pitorre
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Correspondence to Delphine Pitorre.

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Pitorre, D., Llauro, C., Jobet, E. et al. RLK7, a leucine-rich repeat receptor-like kinase, is required for proper germination speed and tolerance to oxidative stress in Arabidopsis thaliana . Planta 232, 1339–1353 (2010). https://doi.org/10.1007/s00425-010-1260-4

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