Abstract
We report isolation and transcriptional profiling of rice (Oryza sativa L.) mitogen-activated protein kinase (MAPK), OsSIPK (salicylic acid-induced protein kinase). OsSIPK gene is located on chromosome 6 most probably existing as a single copy in the rice genome, and encodes 398 amino acid polypeptide having the MAPK family signature and phosphorylation activation motif TEY. Steady state mRNA analyses of OsSIPK showed weak constitutive expression in leaves of 2-week-old rice seedlings. A time course (30–120 min) experiment using a variety of elicitors and stresses revealed that the OsSIPK mRNA is strongly induced by jasmonic acid (JA), salicylic acid (SA), ethephon, abscisic acid, cycloheximide (CHX), JA/SA + CHX, cantharidin, okadaic acid, hydrogen peroxide, chitosan, sodium chloride, and cold stress (12°C), but not with wounding by cut, gaseous pollutants ozone, and sulfur dioxide, high temperature, ultraviolet C irradiation, sucrose, and drought. Its transcription was also found to be tissue-specifically regulated, and followed a rhythmic dark induction in leaves. Finally, we showed that the OsSIPK protein is localized to the nucleus. From these results, OsSIPK can be implicated in diverse stimuli-responsive signaling cascades and transcription of certain genes.
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Abbreviations
- ABA:
-
Abscisic acid
- AtMPK6:
-
Arabidopsis thaliana MAPK6
- AtMPK6 :
-
Arabidopsis thaliana MAPK6 gene
- AtPTP1:
-
Arabidopsis thaliana tyrosine phosphatase 1
- CHX:
-
Cycloheximide
- CRTintP:
-
Calreticulin-interacting protein
- CT:
-
Chitosan
- EN:
-
Endothall
- ET:
-
Ethephon
- FgL:
-
Flag leaf
- FL:
-
First leaf
- HR:
-
Hypersensitive-response
- JA:
-
Jasmonic acid
- L:
-
Leaf
- LS:
-
Leaf sheath
- MAPK:
-
Mitogen-activated protein kinase
- MAPKK:
-
MAPK kinase
- MAPKKK:
-
MAPKK kinase
- MsSIMK:
-
Medicago sativa salt-induced MAPK
- MQ:
-
Milli Q
- NtSIPK:
-
Nicotiana tabacum salicylic acid-induced protein kinase
- OA:
-
Okadaic acid
- OsBIMK1 :
-
Oryza sativa benzothiadiazole-induced MAPK1 gene
- OsBWMK1 :
-
Oryza sativa blast and wound-induced MAPK1 gene
- OsMAPK2 :
-
Oryza sativa MAPK2 gene
- OsMAPK4 :
-
Oryza sativa MAPK4 gene
- OsMAPK6:
-
Oryza sativa MAPK6
- OsMAPK6 :
-
Oryza sativa MAPK6 gene
- OsMSRMK2 :
-
Oryza sativa mutiple stress responsive MAPK2 gene
- OsMSRMK3 :
-
Oryza sativa mutiple stress responsive MAPK3 gene
- OsSIPK:
-
Oryza sativa salicylic acid-induced protein kinase
- OsSIPK :
-
Oryza sativa salicylic acid-induced protein kinase gene
- PAH:
-
Panicles after heading
- PAL:
-
Phenylalanine ammonia-lyase
- PBH:
-
Panicles before heading
- ROS:
-
Reactive oxygen species
- SA:
-
Salicylic acid
- TMV:
-
Tobacco mosaic virus
- UV-C:
-
Ultraviolet C
- WIPK:
-
Wound-inducible protein kinase
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Acknowledgments
The study was funded in part by a grant from the Plant Signaling Network Research Center, Korea Science and Engineering Foundation. The study was also carried out with the support of “On-Site Cooperative Agriculture Research Project (No. 20070301080003), RDA, Republic of Korea (NSJ), and by grant number CG2130 from the Crop Functional Genomics Center of the 21st Century Frontier R&D Program, which is funded by Ministry of Science and Technology of the Republic of Korea. We appreciate a kind gift of rice seeds of the cv. Nipponbare from Dr. Hirohiko Hirochika, Director, Molecular Genetics Department, National Institute of Agrobiological Sciences, Tsukuba, Japan.
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Mi-Ok Lee and Kyoungwon Cho contributed equally to this work.
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Lee, MO., Cho, K., Kim, SH. et al. Novel rice OsSIPK is a multiple stress responsive MAPK family member showing rhythmic expression at mRNA level. Planta 227, 981–990 (2008). https://doi.org/10.1007/s00425-007-0672-2
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DOI: https://doi.org/10.1007/s00425-007-0672-2