Abstract
The aim of the present study was to examine the influence of training state on fasting-induced skeletal muscle pyruvate dehydrogenase (PDH) regulation, including PDH phosphorylation. Trained and untrained subjects, matched for skeletal muscle CS activity and OXPHOS protein, fasted for 36 h after receiving a standardized meal. Respiratory exchange ratio (RER) was measured and blood as well as vastus lateralis muscle biopsies were obtained 2, 12, 24, and 36 h after the meal. RER decreased with fasting only in untrained individuals, while PDHa activity decreased from 12 h after the meal in untrained, but only tended to decrease at 36 h in trained. PDH-E1α, PDP1 protein, PDH phosphorylation, and PDH acetylation in skeletal muscle was higher in trained than untrained subjects, but did not change with fasting, while PDK4 protein was higher at 36 h than at 2 h after the meal in both groups. In conclusion, the present results suggest that endurance exercise training modifies the fasting-induced regulation of PDHa activity in skeletal muscle and the substrate switch towards fat oxidation. PDH phosphorylation could not explain the fasting-induced regulation of PDHa activity suggesting other post translational modifications.
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We would like to thank the involved subjects for participation in the study.
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This study was funded by the Danish Ministry of Culture for Sports Research (1095421001), the Danish Council for Independent Research (36723-104353), and the Danish Diabetes Academy (1105701001). The Centre for Physical Activity Research is supported by a grant from TrygFonden, and the Centre of Inflammation and Metabolism was supported by a grant from the Danish National Research Foundation (DNRF55).
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Gudiksen, A., Bertholdt, L., Stankiewicz, T. et al. Training state and fasting-induced PDH regulation in human skeletal muscle. Pflugers Arch - Eur J Physiol 470, 1633–1645 (2018). https://doi.org/10.1007/s00424-018-2164-6
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DOI: https://doi.org/10.1007/s00424-018-2164-6