Abstract
Ten patients with type 2 diabetes and seven controls were strength-trained with one leg for 30 min three times per week for 6 weeks. The training-induced changes in the protein densities of the Na,K-pump subunits and the Na+/H+ exchanger protein NHE1 were quantified with Western blotting of needle biopsy material obtained from trained and untrained legs of both groups. Training increased the bench press and knee-extensor force by 77±15 and 28±1%, respectively, in the control subjects, and by 75±7 and 42±8%, respectively, in the diabetics. In the control subjects the Na,K-pump isoform α1 was increased by 37% (P<0.05) in trained compared to untrained leg, and in the diabetics the α1 content was 45% higher (P=0.052) in trained compared to untrained leg. For the α2 isoform the corresponding values were 21% and 41% (P<0.05), respectively. The content of the β1 subunit in the control subjects was 33% higher (P<0.05) in trained compared to untrained leg, and 47% higher (P=0.06) in trained compared to untrained leg in the diabetics. Thus, a limited amount of strength-training is able to increase the Na,K-pump subunit and isoform content both in controls and in patients with type 2 diabetes.
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Acknowledgements
The financial support from the Danish Natural Research Foundation, the Danish National Research Foundation (J. no. 504-14), the Danish Diabetes Association, the Novo-Nordisk Foundation, The Foundation of 1870, Jacob and Olga Madsens Foundation, and the Danish Medical Research Council is greatly acknowledged.
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Dela, F., Holten, M. & Juel, C. Effect of resistance training on Na,K pump and Na+/H+ exchange protein densities in muscle from control and patients with type 2 diabetes. Pflugers Arch - Eur J Physiol 447, 928–933 (2004). https://doi.org/10.1007/s00424-003-1213-x
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DOI: https://doi.org/10.1007/s00424-003-1213-x