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Prior cycling exercise does not prevent endothelial dysfunction after resistance exercise

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European Journal of Applied Physiology Aims and scope Submit manuscript

Abstract

Purpose

Resistance exercise impairs endothelial function acutely. Therefore, it becomes important to devise an effective strategy for preventing acute endothelial dysfunction after resistance exercise. Herein, we tested the hypothesis that resistance exercise-induced temporal endothelial dysfunction is prevented by prior cycling.

Methods

Twelve young healthy subjects completed two randomized experimental trials: (1) resistance exercise only trial (RE trial), (2) resistance exercise with prior cycling trial (C + RE trial). Following baseline brachial artery flow-mediated dilation (FMD), the subjects maintained the supine position for 45 min in the RE trial; the subjects performed a 45 min of cycling (67.0 ± 1.7% HRmax) in the C + RE trial. After 45 min of resting or cycling, the subjects performed resistance exercise (69.7 ± 4.0 kg) at the same time points. Following the resistance exercise, they were asked to rest in the supine position for 60 min. Then FMD were repeated at 10, 30 and 60 min after the resistance exercise in both trials.

Results

The increased blood flow and shear rate after resistance exercise did not differ between trials, and these changes disappeared following resting in the supine position for 60 min. There was no significant interaction in %FMD responses. Both trials caused impairment in %FMD after the resistance exercise, and statistical significance was observed at 30 and 60 min after resistance exercise in the RE trial.

Conclusion

The present study revealed that cycling for 45 min prior to resistance exercise was not sufficient to prevent the acute endothelial dysfunction after resistance exercise.

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Abbreviations

FMD:

Flow-mediated dilation

NO:

Nitric oxide

ET-1:

Endothelin-1

1RM:

One repetition maximum

RPE:

Perceived exertion

HR:

Heart rate

AUC:

Area under the curve

ANOVA:

Analysis of variance

ANCOVA:

Analysis of covariance

SE:

Standard error

\(\dot{\text{VO}}_2{\text{max}}\) :

Maximal oxygen uptake

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Acknowledgements

The authors appreciate the time and effort put in by all volunteer subjects. The authors also appreciate Dr. Jaume Padilla for his advice about data analysis.

Funding

This work was supported by The Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research [Grant number 18K17834 (to T.M.), 18K10832 (to E.O.)].

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Authors and Affiliations

  1. Sports Research Center, Hosei University, Tokyo, Japan

    Takuma Morishima & Eisuke Ochi

  2. Faculty of Bioscience and Applied Chemistry, Hosei University, Tokyo, Japan

    Masahira Toyoda & Eisuke Ochi

Authors
  1. Takuma Morishima
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  2. Masahira Toyoda
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  3. Eisuke Ochi
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Contributions

TM and EO: conceptualized and designed the study; TM, MT, and EO: performed the experiments; TM: analyzed data; TM, MT, and EO: interpreted the results of experiments; TM: prepared figures and table. TM and EO: drafted the manuscript; TM, MT, and EO: edited and revised the manuscript; TM, MT, and EO: approved the final version of manuscript.

Corresponding author

Correspondence to Takuma Morishima.

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No conflicts of interest, financial or otherwise, are declared by the authors.

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Communicated by Guido Ferretti.

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Morishima, T., Toyoda, M. & Ochi, E. Prior cycling exercise does not prevent endothelial dysfunction after resistance exercise. Eur J Appl Physiol 119, 1663–1669 (2019). https://doi.org/10.1007/s00421-019-04154-1

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  • DOI: https://doi.org/10.1007/s00421-019-04154-1

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