European Journal of Applied Physiology

, Volume 118, Issue 5, pp 1021–1031 | Cite as

The acute effect of Quercetin on muscle performance following a single resistance training session

  • Federica Patrizio
  • Massimiliano Ditroilo
  • Francesco Felici
  • Guglielmo Duranti
  • Giuseppe De Vito
  • Stefania Sabatini
  • Massimo Sacchetti
  • Ilenia Bazzucchi
Original Article

Abstract

Purpose

To examine the effect of acute quercetin (Q) ingestion on neuromuscular function, biomarkers of muscle damage, and rate of perceived exertion (RPE) in response to an acute bout of resistance training.

Methods

10 young men (22.1 ± 1.8 years, 24.1 ± 3.1 BMI) participated in a randomized, double-blind, crossover study. Subjects consumed Q (1 g/day) or placebo (PLA) 3 h prior to a resistance training session which consisted of 3 sets of 8 repetitions at 80% of the one repetition maximum (1RM) completed bilaterally for eight different resistance exercises. Electromyographic (EMG) signals were recorded from the knee extensor muscles during maximal isometric (MVIC) and isokinetic voluntary contractions, and during an isometric fatiguing test. Mechanical and EMG signals, biomarkers of cell damage, and RPE score were measured PRE, immediately POST, and 24 h (blood indices only) following the resistance exercise.

Results

After a single dose of Q, the torque–velocity curve of knee extensors was enhanced and after the resistance exercise, subjects showed a lower MVIC reduction (Q: 0.91 ± 6.10%, PLA: 8.66 ± 5.08%) with a greater rate of torque development (+ 10.6%, p < 0.005) and neuromuscular efficiency ratio (+ 28.2%, p < 0.005). Total volume of the resistance exercises was significantly greater in Q (1691.10 ± 376.71 kg rep) compared to PLA (1663.65 ± 378.85 kg rep) (p < 0.05) with a comparable RPE score. No significant differences were found in blood marker between treatments.

Conclusions

The acute ingestion of Q may enhance the neuromuscular performance during and after a resistance training session.

Keywords

Flavonoids Strength Electromyography Muscle damage 

Abbreviations

ANOVA

Analysis of variance

BF

Biceps femoris

BMI

Body mass index

CI

Coactivation index

CK

Creatine kinase

EMG

Electromyography

GSH

Glutathione status

GSSG

Oxidized gluthanione

ICC

Intraclass correlation coefficients

IFT

Isometric fatiguing test

MDF

Median frequency

MVIC

Maximal voluntary isometric contraction

NME

Neuromuscular efficiency

PLA

Placebo

PRS

Perceived recovery scale

Q

Quercetin

RF

Rectus femoris

RMS

Root mean square

RPE

Rate of perceived exertion

RTD

Rate of torque development

VAS

Visual analogue scale

1RM

One repetition maximum

References

  1. Aagaard P, Simonsen EB, Andersen JL et al (2002) Increased rate of force development and neural drive of human skeletal muscle following resistance training. J Appl Physiol 93:1318–1326.  https://doi.org/10.1152/japplphysiol.00283.2002 CrossRefPubMedGoogle Scholar
  2. Ackerman J, Clifford T, McNaughton LR, Bentley DJ (2014) The effect of an acute antioxidant supplementation compared with placebo on performance and hormonal response during a high volume resistance training session. J Int Soc Sports Nutr 11:10.  https://doi.org/10.1186/1550-2783-11-10 CrossRefPubMedPubMedCentralGoogle Scholar
  3. Alexander SPH (2006) Flavonoids as antagonists at A1 adenosine receptors. Phyther Res 20:1009–1012.  https://doi.org/10.1002/ptr.1975 CrossRefGoogle Scholar
  4. Andersen LL, Aagaard P (2006) Influence of maximal muscle strength and intrinsic muscle contractile properties on contractile rate of force development. Eur J Appl Physiol 96:46–52.  https://doi.org/10.1007/s00421-005-0070-z CrossRefPubMedGoogle Scholar
  5. Bazzucchi I, Riccio ME, Felici F (2008) Tennis players show a lower coactivation of the elbow antagonist muscles during isokinetic exercises. J Electromyogr Kinesiol 18:752–759CrossRefPubMedGoogle Scholar
  6. Bazzucchi I, Felici F, Sacchetti M (2009) Effect of short-term creatine supplementation on neuromuscular function. Med Sci Sport Exerc.  https://doi.org/10.1249/MSS.0b013e3181a2c05c Google Scholar
  7. Bazzucchi I, Felici F, Montini M et al (2011) Caffeine improves neuromuscular function during maximal dynamic exercise. Muscle Nerve 43:839–844CrossRefPubMedGoogle Scholar
  8. Bazzucchi I, Patrizio F, Felici F et al (2016) CHO mouth rinsing improves neuromuscular performance during isokinetic fatiguing exercise. Int J Sports Physiol Perform ahead of p:1–23.  https://doi.org/10.1123/ijspp.2016-0583
  9. Bloomer RJ, Goldfarb AH, Wideman L et al (2005) Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength Cond Res 19:276.  https://doi.org/10.1519/14823.1 PubMedGoogle Scholar
  10. Boots AW, Haenen GRMM., Bast A (2008) Health effects of quercetin: From antioxidant to nutraceutical. Eur J Pharmacol 585:325–337.  https://doi.org/10.1016/j.ejphar.2008.03.008 CrossRefPubMedGoogle Scholar
  11. Borg GA (1982) Psychophysical bases of perceived exertion. Med Sci Sports Exerc 14:377–381.  https://doi.org/10.1249/00005768-198205000-00012 PubMedGoogle Scholar
  12. Braakhuis AJ, Hopkins WG (2015) Impact of dietary antioxidants on sport performance: a review. Sport Med 45:939–955.  https://doi.org/10.1007/s40279-015-0323-x CrossRefGoogle Scholar
  13. Ceci R, Duranti G, Sgrò P et al (2014) Effects of tadalafil administration on plasma markers of exercise-induced muscle damage, IL6 and antioxidant status capacity. Eur J Appl Physiol 115:531–539.  https://doi.org/10.1007/s00421-014-3040-5 CrossRefPubMedGoogle Scholar
  14. Ceci R, Duranti G, Leonetti A et al (2017) Adaptive responses of heart and skeletal muscle to spermine oxidase overexpression: evaluation of a new transgenic mouse model. Free Radic Biol Med 103:216–225.  https://doi.org/10.1016/j.freeradbiomed.2016.12.040 CrossRefPubMedGoogle Scholar
  15. Cheuvront SN, Ely BR, Kenefick RW et al (2009) No effect of nutritional adenosine receptor antagonists on exercise performance in the heat. Am J Physiol Regul Integr Comp Physiol 65:R394–R401.  https://doi.org/10.1152/ajpregu.90812.2008 CrossRefGoogle Scholar
  16. Chun OK, Chung SJ, Song WO (2007) Estimated dietary flavonoid intake and major food sources of U.S. adults. J Nutr 137:1244–1252 pii]CrossRefPubMedGoogle Scholar
  17. Clifford T, Bell O, West DJ et al (2016) The effects of beetroot juice supplementation on indices of muscle damage following eccentric exercise. Eur J Appl Physiol 116:353–362.  https://doi.org/10.1007/s00421-015-3290-x CrossRefPubMedGoogle Scholar
  18. Connolly DAJ (2006) Efficacy of a tart cherry juice blend in preventing the symptoms of muscle damage * Commentary 1 * Commentary 2. Br J Sports Med 40:679–683.  https://doi.org/10.1136/bjsm.2005.025429 CrossRefPubMedPubMedCentralGoogle Scholar
  19. Davis JM, Murphy EA, Carmichael MD (2009) Effects of the dietary flavonoid quercetin upon performance and health. Curr Sports Med Rep 8:206–213.  https://doi.org/10.1249/JSR.0b013e3181ae8959 CrossRefPubMedGoogle Scholar
  20. Davison G, Callister R, Williamson G et al (2012) The effect of acute pre-exercise dark chocolate consumption on plasma antioxidant status, oxidative stress and immunoendocrine responses to prolonged exercise. Eur J Nutr 51:69–79.  https://doi.org/10.1007/s00394-011-0193-4 CrossRefPubMedGoogle Scholar
  21. De Vito G, McHugh D, Macaluso A, Riches PE (2003) Is the coactivation of biceps femoris during isometric knee extension affected by adiposity in healthy young humans? J Electromyogr Kinesiol 13:425–431.  https://doi.org/10.1016/S1050-6411(03)00061-0 CrossRefPubMedGoogle Scholar
  22. Farina D (2004) The extraction of neural strategies from the surface EMG. J Appl Physiol 96:1486–1495.  https://doi.org/10.1152/japplphysiol.01070.2003 CrossRefPubMedGoogle Scholar
  23. García-Alonso J, Ros G, Vidal-Guevara ML, Periago MJ (2006) Acute intake of phenolic-rich juice improves antioxidant status in healthy subjects. Nutr Res 26:330–339.  https://doi.org/10.1016/j.nutres.2006.06.004 CrossRefGoogle Scholar
  24. Goldfarb AH, Bloomer RJ, Mckenzie MJ (2005) Combined antioxidant treatment effects on blood oxidative stress after eccentric exercise. Med Sci Sports Exerc 37:234–239.  https://doi.org/10.1249/01.MSS.0000152887.87785.BE CrossRefPubMedGoogle Scholar
  25. Goldfarb AH, Garten RS, Cho C et al (2011) Effects of a fruit/berry/vegetable supplement on muscle function and oxidative stress. Med Sci Sports Exerc 43:501–508.  https://doi.org/10.1249/MSS.0b013e3181f1ef48 CrossRefPubMedGoogle Scholar
  26. Harwood M, Danielewska-Nikiel B, Borzelleca JF et al (2007) A critical review of the data related to the safety of quercetin and lack of evidence of in vivo toxicity, including lack of genotoxic/carcinogenic properties. Food Chem Toxicol 45:2179–2205.  https://doi.org/10.1016/j.fct.2007.05.015 CrossRefPubMedGoogle Scholar
  27. Hermens HJ, Freriks B, Disselhorst-Klug C, Rau G (2000) Development of recommendations for SEMG sensors and sensor placement procedures. J Electromyogr Kinesiol 10:361–374.  https://doi.org/10.1016/S1050-6411(00)00027-4 CrossRefPubMedGoogle Scholar
  28. Hollman PCH, Gaag MVD, Mengelers MJB et al (1996) Absorption and disposition kinetics of the dietary antioxidant quercetin in man. Free Radic Biol Med 21:703–707.  https://doi.org/10.1016/0891-5849(96)00129-3 CrossRefPubMedGoogle Scholar
  29. Kale A, Gawande S, Kotwal S et al (2010) Studies on the effects of oral administration of nutrient mixture, quercetin and red onions on the bioavailability of epigallocatechin gallate from green tea extract. Phyther Res.  https://doi.org/10.1002/ptr.2899 Google Scholar
  30. Kaushik D, O’Fallon K, Clarkson PM et al (2012) Comparison of quercetin pharmacokinetics following oral supplementation in humans. J Food Sci doi.  https://doi.org/10.1111/j.1750-3841.2012.02934.x Google Scholar
  31. Kim DH, Ohnishi ST, Ikemoto N (1983) Kinetic studies of calcium release from sarcoplasmic reticulum in vitro. J Biol Chem 258:9662–9668PubMedGoogle Scholar
  32. Konrad M, Nieman DC, Henson DA et al (2011) The acute effect of ingesting a quercetin-based supplement on exercise-induced inflammation and immune changes in runners. Int J Sport Nutr Exerc Metab 21:338–346.  https://doi.org/10.1249/01.MSS.0000402819.42267.6d CrossRefPubMedGoogle Scholar
  33. Larson A, Witman MAH, Guo Y et al (2012) Acute, quercetin-induced reductions in blood pressure in hypertensive individuals are not secondary to lower plasma angiotensin-converting enzyme activity or endothelin-1: Nitric oxide. Nutr Res 32:557–564.  https://doi.org/10.1016/j.nutres.2012.06.018 CrossRefPubMedGoogle Scholar
  34. Lee EH, Meissner G, Kim DH (2002) Effects of quercetin on single Ca2+ release channel behavior of skeletal muscle. Biophys J 82:1266–1277.  https://doi.org/10.1016/S0006-3495(02)75483-0 CrossRefPubMedPubMedCentralGoogle Scholar
  35. MacRae HSH, Mefferd KM (2006) Dietary antioxidant supplementation combined with quercetin improves cycling time trial performance. Int J Sport Nutr Exerc Metab 16:405–419CrossRefPubMedGoogle Scholar
  36. O’Fallon KS, Kaushik D, Michniak-Kohn B et al (2012) Effects of quercetin supplementation on markers of muscle damage and inflammation after eccentric exercise. Int J Sport Nutr Exerc Metab 22:430–437CrossRefPubMedGoogle Scholar
  37. Paulsen G, Hamarsland H, Cumming KT et al (2014) Vitamin C and E supplementation alters protein signalling after a strength training session, but not muscle growth during 10 weeks of training. J Physiol 592:5391–5408.  https://doi.org/10.1113/jphysiol.2014.279950 CrossRefPubMedPubMedCentralGoogle Scholar
  38. Phillips T, Childs AC, Dreon DM et al (2003) A dietary supplement attenuates IL-6 and CRP after eccentric exercise in untrained males. Med Sci Sports Exerc 35:2032–2037.  https://doi.org/10.1249/01.MSS.0000099112.32342.10 CrossRefPubMedGoogle Scholar
  39. Powers SK, Nelson WB, Hudson MB (2011) Exercise-induced oxidative stress in humans: cause and consequences. Free Radic Biol Med 51:942–950.  https://doi.org/10.1016/j.freeradbiomed.2010.12.009 CrossRefPubMedGoogle Scholar
  40. Rainoldi A, Galardi G, Maderna L et al (1999) Repeatability of surface EMG variables during voluntary isometric contractions of the biceps brachii muscle. J Electromyogr Kinesiol 9:105–119.  https://doi.org/10.1016/S1050-6411(98)00042-X CrossRefPubMedGoogle Scholar
  41. Rainoldi A, Gazzoni M, Casale R (2008) Surface EMG signal alterations in carpal tunnel syndrome: a pilot study. Eur J Appl Physiol 103:233–242.  https://doi.org/10.1007/s00421-008-0694-x CrossRefPubMedGoogle Scholar
  42. Remaud A (2005) A methodologic approach for the neuromuscular systemGoogle Scholar
  43. Schoenfeld BJ, Ratamess NA, Peterson MD et al (2014) Effects of different volume-equated resistance training loading strategies on muscular adaptations in well-trained men. J Strength Cond Res 28:2909–2918.  https://doi.org/10.1519/JSC.0000000000000480 CrossRefPubMedGoogle Scholar
  44. Sureda A, Tejada S, Bibiloni M et al (2014) Polyphenols: well beyond the antioxidant capacity: polyphenol supplementation and exercise-induced oxidative stress and inflammation. Curr Pharm Biotechnol 15:373–379.  https://doi.org/10.2174/1389201015666140813123843 CrossRefPubMedGoogle Scholar
  45. Urso ML, Clarkson PM (2003) Oxidative stress, exercise, and antioxidant supplementation. Toxicology 189:41–54.  https://doi.org/10.1016/S0300-483X(03)00151-3 CrossRefPubMedGoogle Scholar
  46. USDA NASS (2009) 2007 Census of Agriculture. 1:p 739Google Scholar
  47. Wu R, Delahunt E, Ditroilo M et al (2016) Effects of age and sex on neuromuscular-mechanical determinants of muscle strength. Age (Omaha).  https://doi.org/10.1007/s11357-016-9921-2 PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Exercise Physiology, Department of Movement, Human and Health SciencesUniversità degli Studi di Roma “Foro Italico”RomaItaly
  2. 2.School of Public Health, Physiotherapy and Sports Science, Institute of Sport and HealthUniversity College DublinDublin 4Ireland
  3. 3.Laboratory of Biochemistry of Movement, Department of Movement, Human and Health SciencesUniversità degli Studi di Roma “Foro Italico”RomaItaly

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