We would like to begin this Editorial for the June issue by acknowledging the pioneering work by Dr. June Almeida and her colleagues who were the first to identify coronaviruses as a class using negative-staining transmission electron microscopy (Almeida et al. 1968). Now, on to this month’s feature articles.
Chemokine signaling and anterior pituitary gland vascular remodeling
The anterior pituitary gland, like other endocrine glands, is rich in fenestrated capillaries (sinusoids) forming an intricate network into which various hormones are released. In addition to five endocrine cell types, the anterior lobe consists of S100β-positive cells as well as endothelial cells and pericytes forming the sinusoids. The majority of the S100β-positive cells are sex-determining region Y-box 2 (SOX2)-positive stem/progenitor cells. Previously, Horiguchi et al. (2012) showed that they may also express CD9 and the CXC chemokine ligand 12 (CXCL12) and its receptor CXCR4. Since chemokines are known to regulate angiogenesis (Strieter et al. 2006; Walenta et al. 2011; Zlotnik and Yoshie 2000), Horiguchi et al. (2020) now investigated by qPCR, ISH and IHC, siRNA knockdown, CD9+ cell isolation, and electron microscopy whether C, CC and CX3C chemokine ligands and their receptors are expressed in the CD9/S100β/SOX2-positive stem/progenitor cells of the anterior lobe (Fig. 1).
They showed the presence of the CX3C chemokine ligand 1 (CXCL1) in 7.8% of the CD9-positive cells. By culturing CD9-positive cells on laminin-coated plates, they could demonstrate their differentiation into endothelial cells, which was accompanied by decreased CX3CL1 and increased endothelial cell progenitor marker Sox18, as well as the CX3CL1 receptor CX3CR1. Similar changes in the expression of these markers were found in vivo during the neovascularization in the prolactinoma rat model. The authors concluded that CX3CL1/CX3CR1 signaling in CD9/S100β/SOX2-positive cells of the anterior lobe plays an important role in resupplying endothelial cells for vascular remodeling.
Cell cycle progression transcriptomic profile of ovarian granulosa cells
In a mature ovarian follicle, granulosa cells (GCs) form the follicle wall and surround oocytes. Besides their architectural tasks, they have important nutritional functions for oocytes during development and maturation (Jankowski et al. 2018). Furthermore, GCs have significant stem cell-like potential and express markers for mesenchymal stem cells and pluripotent stem cells (Kossowska-Tomaszczuk et al. 2009; Varras et al. 2012). Above all, isolated GCs can be grown as monolayer cultures, which can be subjected to molecular analyses. Kulus et al. (2020) report the results of a transcriptomic analysis of the expression of various genes regulating the cell cycle progression in porcine GCs with the use of Affymetrix microarrays (AffymetrixPorcine Gene 1.1 ST Array Strip), which was validated by RT-qPCR. This analysis was accompanied by detailed histological review of ovaries and separated follicles.
Out of 27,558 transcripts analyzed at 48, 96 and 144 h of culture, 3380 transcripts showed a differential expression. These transcripts belonged to 344 Gene Ontology Biological Process (GO BP) terms. For a detailed analysis, the authors focused on “cell cycle checkpoint”, “cell cycle G1/S phase transition”, “cell cycle G2/M phase transition”, “cell cycle phase transition”, “cell cycle process”, “cell cycle” and “cell division” GO BP terms (Fig. 2).
Furthermore, a STRING interaction network for the proteins encoded by the analyzed genes was generated and the functional interactions between selected genes were analyzed. As pointed out by the authors, the large amount of data obtained in this transcriptomic analysis will provide a reference for in vivo studies on the process of mammalian folliculogenesis and future clinical applications of GCs.
“NO skin off my back” to describe this work
Human skin contains a large amount of the small molecule nitric oxide (NO), stored in the more stable forms of nitrite (NO−2), nitrate (NO−3), and S-nitrosothiols (RSNOs), collectively referred to as NOx. Upon stimulation by ultraviolet sunlight, the NOx stores in the skin can be redistributed to the blood stream where they may exert a positive physiological response by lowering vascular blood pressure (Weller 2016). Weller and colleagues have been studying the aspects of this beneficial sunlight-induced process for a number of years (Liu et al. 2014; Mowbray et al. 2009), and have now investigated the most effective range of ultraviolet wavelengths for generating NO (Pelegrino et al. 2020). They used an NO meter with an electrochemical NO sensor to measure the release of free NOx species following irradiation with a range of ultraviolet wavelengths from both a controlled chemical aqueous solution simulacrum and human skin biopsy samples (Fig. 3).
Their results showed that NO generation in human skin samples was most effectively achieved using ultraviolet light with a peak at 280–285 nm (in the UVB range). Moreover, they interestingly found RSNO formation in human skin slices using light at both 320 nm (in the UV region) and 700 nm (in the visible region). These results are of potential interest for clinical trials and photodynamic therapy, but must be measured against the known risk factor of increased exposure to ultraviolet light for developing skin cancer. Ultimately, achieving a balance between the opposing health effects exerted by ultraviolet light on human skin is certainly of great importance moving forward.
“Expansion” of the super-resolution microscopy toolbox
Super-resolution microscopy came to the forefront of imaging in the mid-to-late 2000s in a variety of implementations (Klein et al. 2014; also see the entire Special Issues of Histochemistry and Cell Biology: In Focus: Single-Molecule Super-Resolution Microscopy, June and July 2014; Volume 141, issues 5 and 6). Also referred to as single-molecule imaging, super-resolution microscopy techniques allow sub-diffraction-limited imaging of fluorescently tagged molecules in cells and tissues. To achieve this enhanced resolution, instruments typically consisted of microscopes incorporating powerful lasers for the excitation of a specialized subset of fluorophores, together with a variety of software algorithms for constructing the final image (Klein et al. 2014). Recently, however, Boyden and colleagues have pioneered novel techniques, collectively referred to as “expansion microscopy” (ExM) for achieving super-resolution microscopy using both conventional diffraction-limited microscopy and fluorophores (Alon et al. 2019; Chang et al. 2017; Chen et al. 2015; Gambarotto et al. 2019; Tillberg et al. 2016; Wassie et al. 2019). Other groups have also contributed novel modifications to the expansion microscopy techniques (Li et al. 2018; Truckenbrodt et al. 2018). In essence, expansion microscopy involves introducing hydration-competent polymer gels to physically expand the sample (typically achieving fourfold lateral expansion), resulting in a physical magnification and enhanced super-resolution microscopy (lateral resolution ~ 70 nm) with a diffraction-limited microscope (Chen et al. 2015). Jena and colleagues (Pernal et al. 2020) have now provided additional modifications to the ExM protocol, referred to as “differential expansion microscopy” using rat liver tissue slices and cultured human skeletal muscle cells as samples. With their introduced modified protocol, they were able to achieve greater than 500-fold volumetric sample expansion, and demonstrate anisotropic expansion between tissues, between organelles, and also within organelles themselves (Fig. 4).
They also introduce machine learning approaches based on neural networks for automated processing and analysis of organellar morphometric differences attributable to ExM. Finally, they provide a detailed figure describing their modified expansion protocol, which should be of use for researchers wishing to implement it in their own labs. It is likely that further improvements in expansion and resolution of ExM will be forthcoming in the near future.
Almeida JD, Berry DM, Cunningham CH, Hamre D, Hofstad MS, Mallucci L, McIntosh K, Tyrell DAJ (1968) Nature 220:650
Alon S, Huynh GH, Boyden ES (2019) Expansion microscopy: enabling single cell analysis in intact biological systems. FEBS J 286(8):1482–1494. https://doi.org/10.1111/febs.14597
Chang J-B, Chen F, Yoon Y-G, Jung EE, Babcock H, Kang JS, Asano S, Suk H-J, Pak N, Tillberg PW, Wassie AT, Cai D, Boyden ES (2017) Iterative expansion microscopy. Nat Meth 14(6):593–599. https://doi.org/10.1038/NMETH.4261
Chen F, Tillberg PW, Boyden ES (2015) Expansion microscopy. Science 347(6221):543–548. https://doi.org/10.1126/science.1260088
Gambarotto D, Zwettler FU, Le Guennec M, Schmidt-Cernohorska M, Fortun D, Borgers S, Heine J, Schloetel J-G, Reuss M, Unser M, Boyden ES, Sauer M, Hamel V, Guichard P (2019) Imaging cellular ultrastructures using expansion microscopy (U-ExM). Nat Meth 16:71–74. https://doi.org/10.1038/s41492-018-0238-1
Horiguchi K, Ilmiawati C, Fujiwara K, Tsukada T, Kikuchi M, Yashiro T (2012) Expression of chemokine CXCL12 and its receptor CXCR4 in folliculostellate (FS) cells of the rat anterior pituitary gland: the CXCL12/CXCR4 axis induces interconnection of FS cells. Endocrinology 153:1717–1724
Horiguchi K, Fujiwara K, Yoshida S, Nakakura T, Arae K, Tsukada T, Hasegawa R, Takigami S, Ohsako S, Yashiro T, Kato T, Kato Y (2018) Isolation and characterisation of CD9-positive pituitary adult stem/progenitor cells in rats. Sci Rep 8:5533
Horiguchi K, Fujiwara K, Yoshida S et al (2020) CX3CL1/CX3CR1-signalling in the CD9/S100β/SOX2-positive adult pituitary stem/progenitor cells modulates differentiation into endothelial cells. Histochem Cell Biol. https://doi.org/10.1007/s00418-020-01862-0
Jankowski M, Dyszkiewicz-Konwińska M, Budna J et al (2018) Does migrative and proliferative capability of epithelial cells reflect cellular developmental competence? Med J Cell Biol 6:1–7. https://doi.org/10.2478/acb-2018-0001
Klein T, Proppert S, Sauer M (2014) Eight years of single-molecule localization microscopy. Histochem Cell Biol 141:561–575. https://doi.org/10.1007/s00418-014-1184-3
Kossowska-Tomaszczuk K, De Geyter C, De Geyter M et al (2009) The multipotency of luteinizing granulosa cells collected from mature ovarian follicles. Stem Cells 27:210–219. https://doi.org/10.1634/stemcells.2008-0233
Kulus M, Kranc W, Sujka-Kordowska P et al (2020) Transcriptomic analysis of expression of genes regulating cell cycle progression in porcine ovarian granulosa cells during short-term in vitro primary culture. Histochem Cell Biol. https://doi.org/10.1007/s00418-020-01860-2
Li R, Chen X, Lin Z, Wang Y, Sun Y (2018) Expansion enhanced nanoscopy. Nanoscale 10:17552–17556. https://doi.org/10.1039/cBnr04267e
Liu D, Fernandez BO, Hamilton A, Lang NN, Gallagher JMC, Newby DE, Feelisch M, Weller RB (2014) UVA irradiation of human skin vasodilates arterial vasculature and lowers blood pressure independently of nitric oxide synthase. J Investig Dermatol 134(7):1839–1846
Mowbray M, McLintock S, Weerakoon R, Lomatschinsky N, Jones S, Rossi AG, Weller RB (2009) Enzyme-independent NO stores in human skin: quantification and influence of UV radiation. J Investig Dermatol 129(4):834–842
Pelegrino MT, Paganotti A, Seabra AB, Weller RB (2020) Photochemistry of nitric oxide and S-nitrosothiols in human skin. Histochem Cell Biol. https://doi.org/10.1007/s00418-020-01858-w
Pernal SP, Liyanaarachchi A, Gatti DL, Formosa B, Pulvender R, Kuhn ER, Ramos R, Naik AR, George K, Arslanturk S, Taatjes DJ, Jena BP (2020) Nanoscale imaging using differential expansion microscopy. Histochem Cell Biol. https://doi.org/10.1007/s00418-020-01869-7
Strieter RM, Burdick MD, Mestas J, Gomperts B, Keane MP, Belperio JA (2006) Cancer CXC chemokine networks and tumour angiogenesis. Eur J Cancer 42:768–778
Tillberg PW, Chen F, Piatkevich KD, Zhao Y, Yu C-C, English BP, Gao L, Martorell A, Suk H-J, Yoshida F, DeGennaro EM, Roossien DH, Gong G, Seneviratne U, Tannenbaum SR, Desimone R, Cai D, Boyden ES (2016) Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nat Biotechnol 34(9):987–992. https://doi.org/10.1038/nbt.3625
Truckenbrodt S, Maidorn M, Crzan D, Wildhagen H, Kabatas S, Rizzoli SO (2018) X10 expansion microscopy enables 25-nm resolution on conventional microscopes. EMBO Rep 19:e45836. 10.15252/embr.201845836
Varras M, Griva T, Kalles V et al (2012) Markers of stem cells in human ovarian granulosa cells: is there a clinical significance in ART? J Ovarian Res 5:36. https://doi.org/10.1186/1757-2215-5-36
Walenta KL, Bettink S, Bohm M, Friedrich EB (2011) Differential chemokine receptor expression regulates functional specialization of endothelial progenitor cell subpopulations. Basic Res Cardiol 106:299–305
Wassie AT, Zhao Y, Boyden ES (2019) Expansion microscopy: principles and uses in biological research. Nat Meth 16:33–41. https://doi.org/10.1038/s41592-018-0219-4
Weller RB (2016) Sunlight has cardiovascular benefits independently of vitamin D. Blood Purif 41(1–3):130–134
Zlotnik A, Yoshie O (2000) Chemokines: a new classification system and their role in immunity. Immunity 12:121–127
News from the Society for Histochemistry
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Taatjes, D.J., Roth, J. In focus in HCB. Histochem Cell Biol 153, 379–384 (2020). https://doi.org/10.1007/s00418-020-01885-7