Histochemistry and Cell Biology

, Volume 149, Issue 4, pp 417–422 | Cite as

In vivo live imaging of bone cells



There are as many as 200 cell types in the body, and highly sophisticated and varied life phenomena are carried out by cell migration to appropriate places at appropriate times following the appropriate interactions. Recent advances in optical imaging technology using multi-photon excitation microscopy have enabled visualization inside intact bone tissues in living animals without thin sectioning. Using such advanced techniques, the dynamic behaviors of living bone cells on intact bone tissue structures can be elucidated. Here, we focus on recent findings using intravital multi-photon imaging of dynamic biological systems, e.g., bone homeostasis. This novel approach has proven beneficial for understanding the mechanisms underlying the spatiotemporal nature of bone remodeling systems and for evaluating the specific modes of actions of novel drugs currently in development, which will contribute to a new chapter in bone and mineral research.


Intravital imaging Multi-photon microscopy Cellular dynamics Bone Osteoclast pH probe 



This work was supported by CREST, Japan Science and Technology Agency, and Grants-in-Aid for Scientific Research (A) from the Japan Society for the Promotion of Science (JSPS to M.I.); Grant-in-Aid for Young Scientists (A) from JSPS (to J.K.); and Grant-in-Aid for Young Scientists (B) from JSPS (to H.M.).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, and WPI-Immunology Frontier Research CenterOsaka UniversitySuitaJapan

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