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17β-estradiol mediates upregulation of stromal cell-derived factor-1 in the retina through activation of estrogen receptor in an ischemia-reperfusion injury model

  • Retinal Disorders
  • Published:
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Abstract

Purpose

There is increasing evidence that suggests stromal cell-derived factor-1 (SDF-1) can induce a protective response against ischemic injury in various organs. In this study, we examined the expression of SDF-1 in a rat model of retinal ischemia-reperfusion (IR) injury. Further, we explored the effect of estrogen 17β-estradiol (E2), and the role of estrogen receptor (ER) in regulating SDF-1 expression.

Methods

Retinal IR injury was established in Sprague–Dawley rats by elevating the intraocular pressure to 110 mmHg for 60 mins. Relative expression levels of SDF-1 mRNA and protein in the retina at 6 h, 12 h, and 24 h after reperfusion were determined by RT-PCR and western blot respectively. To investigate the influence of estrogen and ER on SDF-1 expression, E2 was administered intraperitoneally 30 mins before induction of ischemia, and the estrogen receptor antagonist ICI 182–780 was administered 1 h before E2 injection.

Results

SDF-1 expression in IR-injured retina is upregulated at 6 h, 12 h, and 24 h after injury, with maximum expression at 12 h. As expected, pretreatment of retinal IR rats with E2 enhanced the upregulation in SDF-1 expression after injury, through activation of the estrogen receptor. We proved this hypothesis by demonstrating that pretreatment of retinal IR rats with ICI 182–780 led to a partial decrease in E2-induced SDF-1 expression.

Conclusions

Our findings suggest that 17β-estradiol offers protection against retinal ischemic injury by inducing an upregulation in SDF-1 expression through activation of the estrogen receptor.

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Acknowledgments

This work was supported by Foundation of Heilongjiang Educational Department (NO. 12531389).

All authors have read and approved the content, and agree to submit for consideration for publication in the journal. There are no any ethical/legal conflicts involved in the article.

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Authors and Affiliations

  1. Department of Ophthalmology, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin, Heilongjiang Province, People’s Republic of China, 150001

    Yeqing Wang, Jian Wang, Huanqi Shi, Wenjiao Bi, Wenwen Hou & Xiaomei Zhang

  2. College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, Heilongjiang Province, People’s Republic of China, 150081

    Xia Li

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  1. Yeqing Wang
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  2. Xia Li
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  3. Jian Wang
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  7. Xiaomei Zhang
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Correspondence to Xiaomei Zhang.

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Wang, Y., Li, X., Wang, J. et al. 17β-estradiol mediates upregulation of stromal cell-derived factor-1 in the retina through activation of estrogen receptor in an ischemia-reperfusion injury model. Graefes Arch Clin Exp Ophthalmol 253, 17–23 (2015). https://doi.org/10.1007/s00417-014-2657-8

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  • DOI: https://doi.org/10.1007/s00417-014-2657-8

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