Abstract
In order to determine the population allele frequencies of autosomal STR markers of forensic interest in the Zimbabwean population, we analyzed a sample of 478 individuals from 19 different ethnic groups using the PowerPlex® Fusion 6C Kit (Promega Corp, Madison, Wisconsin). The data obtained were compared among the different Zimbabwean ethnic groups as well as with several African populations to establish whether significant differences exist among them. No significant differences were found among the ethnic groups in Zimbabwe. Statistically significant differences were observed between allele frequencies in Zimbabwe and some other African populations, although FST with neighboring Bantu populations from South and Southeast regions were low (below 0.005 in most single locus comparisons).
Similar content being viewed by others
References
Budowle B, Nhari LT, Moretti TR, Kanoyangwa SB, Masuka E, Defenbaugh DA, Smerick JB (1997) Zimbabwe black population data on the six short tandem repeat loci--CSF1PO, TPOX, THO1, D3S1358, VWA and FGA. FSI 90:215–221
World Medical Association (2013) World Medical Association Declaration of Helsinki: Ethical Principles for Medical Research Involving Human Subjects. JAMA 310:2191–2194
Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinformatics Online 1:47–50
Weir BS (1996) Genetic Data Analysis II: Methods for Discrete Population Genetic Data. Sinauer Associates, USA, pp 109–110
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Felsenstein J (2005) PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle
Page RDM (1996) Treeview: An application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
Lane AB (2008) The nature of tri-allelic TPOX genotypes in African populations. Forensic Sci Int Genet 2:134–137
Melo MM, Carvalho M, Lopes V, Anjos MJ, Serra A, Vieira DN, Sequeiros J, Corte-Real F, Melo MM (2010) Genetic study of 15 STRs loci of Identifiler system in Angola population. Forensic Sci Int Genet 4:e153–e157
Beleza S, Alves C, Reis F, Amorim A, Carracedo A, Gusmao L (2004) 17 STR data (AmpF/STR Identifiler and Powerplex 16 System) from Cabinda (Angola). Forensic Sci Int 141:193–196
Tau T, Wally A, Fanie TP, Ngono GN, Mpoloka SW, Davison S, D’Amato ME (2017) Genetic variation and population structure of Botswana populations as identified with AmpFLSTR Identifiler short tandem repeat (STR) loci. Sci Rep 7:6768
Alves C, Gusmão L, López-Parra AM, Soledad Mesa M, Amorim A, Arroyo-Pardo E (2005) STR allelic frequencies for an African population sample (Equatorial Guinea) using AmpFlSTR Identifiler and Powerplex 16 kits. Forensic Sci Int 148:239–242
Calzada P, Suárez I, García S, Barrot C, Sánchez C, Ortega M, Mas J, Huguet E, Corbella J, Gené M (2005) The Fang population of Equatorial Guinea characterised by 15 STR-PCR polymorphisms. Int J Legal Med 119:107–110
Gonçalves R, Jesus J, Fernandes AT, Brehm A (2002) Genetic profile of a multi-ethnic population from Guiné-Bissau (west African coast) using the new PowerPlex® 16 System kit. Forensic Sci Int 129:78–80
Alves C, Gusmão L, Damasceno A, Soares B, Amorim A (2004) Contribution for an African autosomic STR database (AmpF/STR Identifiler and Powerplex 16 System) and a report on genotypic variations. Forensic Sci Int 139:201–205
Muro T, Fujihara J, Imamura S, Nakamura H, Yasuda T, Takeshita H (2008) Allele frequencies for 15 STR loci in Ovambo population using AmpFlSTR Identifiler Kit. Legal Med 10:157–159
Okolie V, Cisana S, Schanfield MS, Adekoya KO, Oyedeji OA, Podini D (2018) Population data of 21 autosomal STR loci in the Hausa, Igbo and Yoruba people of Nigeria. Int J Legal Med 132:735–737
Tofanelli S, Boschi I, Bertoneri S, Coia V, Taglioli L, Franceschi MG, Destro-Bisol G, Pascali V, Paoli G (2003) Variation at 16 STR loci in Rwandans (Hutu) and implications on profile frequency estimation in Bantu-speakers. Int J Legal Med 117:121–126
Regueiro M, Carril JC, Pontes ML, Pinheiro MF, Luis JR, Caeiro B (2004) Allele distribution of 15 PCR-based loci in the Rwanda Tutsi population by multiplex amplification and capillary electrophoresis. Forensic Sci Int 143:61–63
Tillmar AO, Bäckström G, Montelius K (2009) Genetic variation of 15 autosomal STR loci in a Somali population. Forensic Sci Int Genet 4:e19–e20
Ristow PG, Cloete KW, D'Amato ME (2016) GlobalFiler® Express DNA amplification kit in South Africa: Extracting the past from the present. Forensic Sci Int Genet 24:194–201
Lucassen A, Ehlers K, Grobler PJ, Shezi AL (2014) Allele frequency data of 15 autosomal STR loci in four major population groups of South Africa. Int J Legal Med 128:275–276
Forward BW, Eastman MW, Nyambo TB, Ballard RE (2008) AMPFlSTRR IdentifilerTM STR Allele Frequencies in Tanzania, Africa. J Forensic Sci 53(1):245–247
Gomes V, Sánchez-Diz P, Alves C, Gomes I, Amorim A, Carracedo A, Gusmão L (2009) Population data defined by 15 autosomal STR loci in Karamoja population (Uganda) using AmpF/STR Identifiler kit. Forensic Sci Int Genet 3:e55–e58
Funding
LG was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq (ref. 306342/2019-7), and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro – FAPERJ (CNE-2018).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Informed consent
This study followed the ethical principles of the 2000 Helsinki Declaration of the World Medical Association and written informed consent was obtained from the participants for cooperation under strictly confidential conditions.
Additional information
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Borosky, A., Rotondo, M., Eppel, S. et al. Allele frequency data for 23 aSTR for different ethnic groups from Republic of Zimbabwe. Int J Legal Med 135, 1753–1765 (2021). https://doi.org/10.1007/s00414-021-02514-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00414-021-02514-1