Evaluation of genetic polymorphism at 20 autosomal STR loci in Rajput population of Himachal Pradesh, India


In the present study, genetic diversity indices and forensic parameters of Rajput population of Himachal Pradesh, India, were obtained at 20 autosomal short tandem repeat (STR) loci included in the PowerPlex® 21 System (Promega, USA). With 227 alleles and 11.35 ± 0.89 alleles per locus, none of the locus deviated from the Hardy–Weinberg equilibrium (HWE). The most polymorphic and discriminative locus was Penta E. Phylogenetic tree showed high genetic affinity of Rajput population of Himachal Pradesh with Rajputs of Madhya Pradesh and Yadav of Bihar. Overall, the tested 20 autosomal STRs were polymorphic in Rajput population and found suitable in forensic casework and population genetic studies.

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We thank all individuals who generously donated samples to undertake this study.

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Correspondence to Vivek Sahajpal or Mukesh Thakur.

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The study was conducted in compliance with the ethical standards. All the procedures prior to their implementation were approved by the Ethical Committee of State Forensic Science Laboratory, Directorate of Forensic Services, Shimla, Himachal Pradesh.

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The authors declare that they have no conflict of interest.

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Sahajpal, V., Singh, A., Thakur, M. et al. Evaluation of genetic polymorphism at 20 autosomal STR loci in Rajput population of Himachal Pradesh, India. Int J Legal Med 134, 1323–1324 (2020). https://doi.org/10.1007/s00414-019-02198-8

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  • PowerPlex® 21 System (Promega, USA)
  • Rajput population
  • Himachal Pradesh
  • Population genetic analysis
  • STRs
  • DNA Technology (Use and Application) Regulation Bill, 2019