Forensic effectiveness and genetic distribution of 20 non-CODIS STRs and D3S1358 in Guangdong Han population

Abstract

In this study, Guangdong Han population was analyzed with Goldeneye™ DNA ID 22NC Kit containing 20 non-CODIS STR loci and a CODIS STR locus. In 503 unrelated individuals, a total of 232 alleles and 813 genotypes were observed. The values of polymorphism information content (PIC), expected heterozygosity (He), and observed heterozygosity (Ho) ranged from 0.6678 to 0.8618, 0.7197 to 0.8757, and 0.6938 to 0.8767, respectively. No deviation from Hardy-Weinberg equilibrium was observed at all loci after the Bonferroni correction. The combined power of discrimination and the combined power of exclusion were 0.999999999999999999999999937051 and 0.999999998467033, respectively. Population comparison revealed that Guangdong Han population was relatively close to Huadong Han population. These results suggested that the 21 STR loci are highly polymorphic and Goldeneye™ DNA ID 22NC Kit is suitable for individual identification and paternity testing in Guangdong Han population.

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References

  1. 1.

    Guangdong Provincial Bureau of Statistics 2015 Bulletin of the main data of the 1% national sample survey of Guangdong Province in. http://www.gdstats.gov.cn/tjzl/tjgb/201605/t20160511_327841.html,2016-05-10

  2. 2.

    Guangdong provincial local history office (n.d.) History of population. http://www.gd-info.gov.cn/books/dtree/showbook.jsp?stype=v&paths=10865&siteid=guangdong&sitename=%E5%B9%BF%E4%B8%9C%E7%9C%81%E6%83%85%E7%BD%91

  3. 3.

    Wu J (2005) Discussion on the characteristics and countermeasures of population migration and flow in Guangdong Province. South China Journal of Economics 02:55–57

    Google Scholar 

  4. 4.

    Du W, Wu W, Wu Z et al (2019) Genetic polymorphisms of 32 Y-STR loci in Meizhou Hakka population. Int J Legal Med 133(2):465–466

    Article  Google Scholar 

  5. 5.

    Lu H, Qiu P, Liu C et al (2017) Genetic polymorphism of 21 non-CODIS STR loci for Guangdong (southern China) Han population. Forensic Science International: Genetics 27:180–181

    CAS  Article  Google Scholar 

  6. 6.

    Zhu BF, Zhang YD, Shen CM et al (2015) Developmental validation of the AGCU 21+1 STR kit: a novel multiplex assay for forensic application. Electrophoresis 36:271–276

    CAS  Article  Google Scholar 

  7. 7.

    Yuan LIN, Ting-zhi QUE, Zhenmin ZHAO et al (2015) Forensic investigation of goldeneye™ DNA ID 22NC kit. Journal of Forensic Medicine 31(4):280–283

    CAS  Google Scholar 

  8. 8.

    Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under linux and windows. Mol Ecol Resour 10:564–567

    Article  Google Scholar 

  9. 9.

    Hammer Ø, Harper D, Ryan P (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron 4

  10. 10.

    Bland JM, Altman DG (1995) Multiple significance tests: the Bonferroni method. BMJ 310:170

    CAS  Article  Google Scholar 

  11. 11.

    Fu X, He J, Guo J et al (2017) Genetic distribution of 21 STR loci in the Chinese Han population from Hainan Island using Goldeneye™ DNA ID 22NC Kit. Forensic Science International: Genetics 29:19–20

    Article  Google Scholar 

  12. 12.

    Fu X, Liu Y, Wang Z et al (2017) Genetic polymorphisms of 21 STR loci in Goldeneye™ DNA ID 22NC Kit for Chinese Li ethnic group. Forensic Science International: Genetics 28:45–46

    Article  Google Scholar 

  13. 13.

    Gou H, Zhou J, Zhang Y et al (2016) Allele frequency distribution of 21 forensic autosomal STR loci of Goldeneye™ DNA ID 22NC Kit in Chinese Tibetan group. Forensic Science International: Genetics 22:21–24

    Article  Google Scholar 

  14. 14.

    Fu Y, Liu H (2017) Goldeneye™ DNA identification system 22NC forensic investigation of Hui population. Journal of Forensic Medicine 33(3):304–306

    Google Scholar 

  15. 15.

    Song X, Gou H, Zhong H et al (2018) Genetic polymorphisms of 21 autosomal STR loci in Chinese Uygur. Int J Legal Med 132:1637–1639

    Article  Google Scholar 

Download references

Funding

This work was financially supported by a grant from the Natural Science Foundation of Guangdong Province (Grant no. 2014A030310025).

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Correspondence to Ling Chen.

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The authors declare that they have no conflict of interest.

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• Goldeneye™ DNA ID 22NC Kit includes 20 non-CODIS STR loci and a CODIS STR locus.

• Goldeneye™ DNA ID 22NC Kit was firstly used to detect the genotypes and study the genetic polymorphism of the Guangdong Han population.

• Guangdong Han population has relatively close genetic relationship with the Huadong Han population.

• Goldeneye™ DNA ID 22NC Kit could be used for individual identification and paternity testing.

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Peng, H., Li, X., Yao, T. et al. Forensic effectiveness and genetic distribution of 20 non-CODIS STRs and D3S1358 in Guangdong Han population. Int J Legal Med 134, 1319–1321 (2020). https://doi.org/10.1007/s00414-019-02094-1

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Keywords

  • Population polymorphism
  • Short tandem repeats
  • Guangdong Han
  • Goldeneye™ DNA ID 22NC kit