The Qiagen Investigator® DIPplex Kit multiplexes 30 autosomal INDELs plus amelogenin for forensic use. The aim of this study was to estimate the diversity of 30 INDEL loci in a sample of 530 Tibetan individuals from Qinghai Province, China, and to evaluate the usefulness of these loci for forensic genetics. The observed heterozygosity ranged from 14.3 to 53.4%, and combined power of discrimination, matching probability, and cumulative probability of exclusion were 0.99999999999172, 8.27999 × 10−12, and 0.9897, respectively, in the Qinghai Tibetan group. The results of pairwise genetic distance, principal component analysis, a multi-dimensional scaling plot, and a neighbour-joining tree between the Qinghai Tibetan and 49 reference populations revealed significant genetic differences between continental populations and a close genetic relationship between the Qinghai Tibetan and East Asian populations. This study indicates that the Investigator® DIPplex Kit can serve as an effective supplementary tool for forensic genetic tasks.
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We thank all sample donors for their contributions to this work and all those who helped with sample collection.
This work received financial support from the Department of Science & Technology of Sichuan Province (2017JY0233).
Conflict of interest
The authors declare that they have no conflict of interest.
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Jian, H., Wang, L., Wang, H. et al. Population genetic analysis of 30 insertion–deletion (INDEL) loci in a Qinghai Tibetan group using the Investigator DIPplex Kit. Int J Legal Med 133, 1039–1041 (2019). https://doi.org/10.1007/s00414-018-1954-x
- Investigator® DIPplex kit
- Qinghai Tibetan
- Forensic genetics
- Population study