Population genetic analysis of 30 insertion–deletion (INDEL) loci in a Qinghai Tibetan group using the Investigator DIPplex Kit


The Qiagen Investigator® DIPplex Kit multiplexes 30 autosomal INDELs plus amelogenin for forensic use. The aim of this study was to estimate the diversity of 30 INDEL loci in a sample of 530 Tibetan individuals from Qinghai Province, China, and to evaluate the usefulness of these loci for forensic genetics. The observed heterozygosity ranged from 14.3 to 53.4%, and combined power of discrimination, matching probability, and cumulative probability of exclusion were 0.99999999999172, 8.27999 × 10−12, and 0.9897, respectively, in the Qinghai Tibetan group. The results of pairwise genetic distance, principal component analysis, a multi-dimensional scaling plot, and a neighbour-joining tree between the Qinghai Tibetan and 49 reference populations revealed significant genetic differences between continental populations and a close genetic relationship between the Qinghai Tibetan and East Asian populations. This study indicates that the Investigator® DIPplex Kit can serve as an effective supplementary tool for forensic genetic tasks.

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  • 26 February 2020

    Concerns have been raised about the ethics approval and informed consent procedures related to the research reported in this paper. The paper includes the following author declarations: “Written informed consent was obtained from all the participants”. Editorial action will be taken as appropriate once an investigation of the concerns is complete and all parties have been given an opportunity to respond in full.


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We thank all sample donors for their contributions to this work and all those who helped with sample collection.


This work received financial support from the Department of Science & Technology of Sichuan Province (2017JY0233).

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Correspondence to Meili Lv or Weibo Liang.

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Jian, H., Wang, L., Wang, H. et al. Population genetic analysis of 30 insertion–deletion (INDEL) loci in a Qinghai Tibetan group using the Investigator DIPplex Kit. Int J Legal Med 133, 1039–1041 (2019). https://doi.org/10.1007/s00414-018-1954-x

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  • Investigator® DIPplex kit
  • Qinghai Tibetan
  • Forensic genetics
  • Population study