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Population genetic analysis of 30 insertion–deletion (INDEL) loci in a Qinghai Tibetan group using the Investigator DIPplex Kit

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26 February 2020 Concerns have been raised about the ethics approval and informed consent procedures related to the research reported in this paper. The paper includes the following author declarations: “Written informed consent was obtained from all the participants”. Editorial action will be taken as appropriate once an investigation of the concerns is complete and all parties have been given an opportunity to respond in full.

Abstract

The Qiagen Investigator® DIPplex Kit multiplexes 30 autosomal INDELs plus amelogenin for forensic use. The aim of this study was to estimate the diversity of 30 INDEL loci in a sample of 530 Tibetan individuals from Qinghai Province, China, and to evaluate the usefulness of these loci for forensic genetics. The observed heterozygosity ranged from 14.3 to 53.4%, and combined power of discrimination, matching probability, and cumulative probability of exclusion were 0.99999999999172, 8.27999 × 10−12, and 0.9897, respectively, in the Qinghai Tibetan group. The results of pairwise genetic distance, principal component analysis, a multi-dimensional scaling plot, and a neighbour-joining tree between the Qinghai Tibetan and 49 reference populations revealed significant genetic differences between continental populations and a close genetic relationship between the Qinghai Tibetan and East Asian populations. This study indicates that the Investigator® DIPplex Kit can serve as an effective supplementary tool for forensic genetic tasks.

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  • 26 February 2020

    Concerns have been raised about the ethics approval and informed consent procedures related to the research reported in this paper. The paper includes the following author declarations: “Written informed consent was obtained from all the participants”. Editorial action will be taken as appropriate once an investigation of the concerns is complete and all parties have been given an opportunity to respond in full.

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Acknowledgments

We thank all sample donors for their contributions to this work and all those who helped with sample collection.

Funding

This work received financial support from the Department of Science & Technology of Sichuan Province (2017JY0233).

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Author notes

  1. Hui Jian and Li Wang contributed equally to this work.

Authors and Affiliations

  1. Department of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China

    Hui Jian & Weibo Liang

  2. Department of Obstetrics and Gynecology, Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University) of Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China

    Li Wang

  3. Chengdu Public Security Bureau, Institute of Forensic Science, Chengdu, 610081, Sichuan, China

    Hui Wang & Xiaogang Bai

  4. Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China

    Meili Lv

Authors
  1. Hui Jian
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  2. Li Wang
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  3. Hui Wang
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  4. Xiaogang Bai
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Corresponding authors

Correspondence to Meili Lv or Weibo Liang.

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The authors declare that they have no conflict of interest.

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Jian, H., Wang, L., Wang, H. et al. Population genetic analysis of 30 insertion–deletion (INDEL) loci in a Qinghai Tibetan group using the Investigator DIPplex Kit. Int J Legal Med 133, 1039–1041 (2019). https://doi.org/10.1007/s00414-018-1954-x

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  • DOI: https://doi.org/10.1007/s00414-018-1954-x

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