Abstract
Mexican Mestizos (admixed) have been poorly studied for short tandem repeats (STRs) included for new human identification (HID) kits, such as the GlobalFiler PCR Amplification kit. Therefore, this kit was analyzed in 784 unrelated volunteers from the city of Tijuana (n = 381) and Sonora state (n = 403) in the northwest region of Mexico. Allele frequencies, forensic parameters, Hardy–Weinberg equilibrium, and linkage equilibrium were estimated or evaluated for 21 autosomal STRs, respectively. For this HID kit, the combined power of discrimination (PD) was > 0.99999999999999 (RMP range = 1.23 to 3.0 × 10−25), and the combined power of exclusion (PE) were 0.999999993 and 0.999999997 in Tijuana city and Sonora state, respectively. Interpopulation analyses based on STRs of the GlobalFiler kit was performed, including four Mexican Native American, one Mexican Mestizo, and four ethnic American populations (USA), previously studied. The low—but significant—differentiation observed among Mexican Mestizos (FST = 0.0969%; p = 0.02584) justifies the creation of STR databases for HID purposes in this country. In brief, results allow the confident use of the GlobalFiler kit for HID purposes in Mestizo population from the Northwest region Mexico.
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References
Rubi-Castellanos R, Martinez-Cortes G, Munoz-Valle JF, Gonzalez-Martin A, Cerda-Flores RM, Anaya-Palafox M, Rangel-Villalobos H (2009) Pre-Hispanic Mesoamerican demography approximates the present-day ancestry of Mestizos throughout the territory of Mexico. Am J Phys Anthropol 139(3):284–294
Salazar-Flores J, Zuniga-Chiquette F, Rubi-Castellanos R, Alvarez-Miranda JL, Zetina-Hernandez A, Martinez-Sevilla VM, Gonzalez-Andrade F, Corach D, Vullo C, Alvarez JC, Lorente JA, Sanchez-Diz P, Herrera RJ, Cerda-Flores RM, Munoz-Valle JF, Rangel-Villalobos H (2015) Admixture and genetic relationships of Mexican Mestizos regarding Latin American and Caribbean populations based on 13 CODIS-STRs. Homo 66(1):44–59
Ramos-González B, Aguilar-Velázquez JA, Chávez-Briones ML, Delgado-Chavarría JR, Alfaro-Lopez E, Rangel-Villalobos H (2016) Population data of 24 STRs in Mexican-Mestizo population from Monterrey, Nuevo Leon (northeast, Mexico) based on Powerplex® Fusion and GlobalFiler® kits. Forensic Sci Int Genet 21:e15–e17
Moreno-Estrada A, Gignoux CR, Fernández-López JC, Zakharia F, Sikora M, Contreras AV, Acuña-Alonzo V, Sandoval K, Eng C, Romero-Hidalgo S, Ortiz-Tello P, Robles V, Kenny EE, Nuño-Arana I, Barquera-Lozano R, Macín-Pérez G, Granados-Arriola J, Huntsman S, Galanter JM, Via M, Ford JG, Chapela R, Rodriguez-Cintron W, Rodríguez-Santana JR, Romieu I, Sienra-Monge JJ, del Rio Navarro B, London SJ, Ruiz-Linares A, Garcia-Herrera R, Estrada K, Hidalgo-Miranda A, Jimenez-Sanchez G, Carnevale A, Soberón X, Canizales-Quinteros S, Rangel-Villalobos H, Silva-Zolezzi I, Burchard EG, Bustamante CD (2014) The genetics of Mexico recapitulates Native American substructure and affects biomedical traits. Science 344(6189):1280–1285
GlobalFiler™ PCR Amplification kit user guide (pub. No. 4477604). https://tools.thermofisher.com/content/sfs/manuals/4477604. PDF: Applied Biosystems
Tereba A (1999) Tools for analysis of population statistics. Profiles in DNA 3:14–16
National Research Council (NRC) Committee on DNA Forensic Science (1996) The evaluation of forensic DNA evidence. National Academic Press, Washington, D.C
Lewis PO, Zaykin D (2015) Genetic data analysis (GDA): computer program for the analysis of allelic data v.1.1. https://phylogeny.uconn.edu/software/
Excoffier L, Lischer H (2010) Arlequin suite ver3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10(3):564–567
Hill CR, Duewer DL, Kline MC, Coble MD, Butler JM (2013) U.S. population data for 29 autosomal STR loci. Forensic Sci Int Genet 7:e82–e83
Ng J, Oldt RF, McCulloh KL, Weise JA, Viray J, Budowle B, Smith DG, Kanthaswamy S (2016) Native American population data based on the GlobalFiler® autosomal STR loci. Forensic Sci Int Genet 24:e12–e13
Page RD (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12(4):357–358
IBM (2010) SPSS Statistics for Windows, 19.0 edn. IBM Corp, Armonk
Rangel-Villalobos H, Martinez-Sevilla VM, Martínez-Cortes G, Aguilar-Velázquez JA, Sosa-Macias M, Rubi-Castellanos R, Gonzalez-Martin A (2016) Importance of the geographic barriers to promote gene drift and avoid (pre and post-Columbian) gene flow in Mexican Native groups: evidence from forensic STR loci. Am J Phys Anthropol 160(2):298–316
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Martínez-Cortés, G., Zuñiga-Chiquette, F., Celorio-Sánchez, A.S. et al. Population data for 21 autosomal STR loci (GlobalFiler kit) in two Mexican-Mestizo population from the northwest, Mexico. Int J Legal Med 133, 781–783 (2019). https://doi.org/10.1007/s00414-018-1950-1
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DOI: https://doi.org/10.1007/s00414-018-1950-1