Abstract
Low levels of DNA from an unidentified human source, often referred to as trace DNA, are ubiquitous, can be transferred onto objects by either direct or indirect methods and have an unknown longevity in situ. Clothing items from crime scenes are often submitted for trace DNA analysis, usually in attempt to identify a person of interest. This study examined the transfer of DNA onto three 10 × 10 cm areas located on the front, back and shoulder of an individual’s external clothing (n = 300) during a regular day’s activity. After wearing for a day, the DNA quantity on all three areas increased approximately 8-fold, which usually corresponded with an increase in the endogenous DNA from the wearer on the front area of the shirt. However, the back area of the shirt was more likely to demonstrate mixtures of endogenous and extraneous DNA. An additional study was also carried out to examine whether domestic laundering is a possible mechanism for the transfer of foreign DNA onto freshly laundered items and revealed that 74% of UV-treated cotton swatch samples produced DNA profiles after laundry with household garments. In summary, this study highlights the ease of DNA transfer onto an individual’s external clothing during a regular day, and that extraneous DNA may be already on the clothing item prior to it being worn. The study provides empirical data to assist in the interpretation of trace DNA profiles and support a Bayesian approach to estimate statistical likelihoods for the transfer of foreign DNA.
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References
Wickenheiser R (2002) Trace DNA: a review, discussion of theory, and application of the transfer of trace quantities of DNA through skin contact. J Forensic Sci 47(3):442–450
Phipps M, Petricevic S (2007) The tendency of individuals to transfer DNA to handled items. Forensic Sci Int 168(2-3):162–168. https://doi.org/10.1016/j.forsciint.2006.07.010
Goray M, Van Oorschot R (2015) The complexities of DNA transfer during social setting. Legal Med 17(2):82–91. https://doi.org/10.1016/j.legalmed.2014.10.003
Van Oorschot R, Glavich G, Mitchell J (2014) Persistence of DNA deposited by the original user on objects after subsequent use by a second person. Forensic Sci Int Genet 8(1):219–225. https://doi.org/10.1016/j.fsigen.2013.10.005
Van Oorschot R, Goray M, Eken E, Mitchell R (2009) Impact of relevant variables on the transfer of biological substances. Forensic Sci Int Genet Suppl Ser 2(1):547–548. https://doi.org/10.1016/j.fsigss.2009.08.105
Meakin G, Jamieson A (2013) DNA transfer: review and implications for casework. Forensic Sci Int Genet 7(4):434–443. https://doi.org/10.1016/j.fsigen.2013.03.013
Goray M, Mitchell J, Van Oorschot R (2011) Evaluation of multiple transfer of DNA using mock case scenarios. Legal Med 14(1):40–46. https://doi.org/10.1016/j.legalmed.2011.09.006
Van den Berge M, Ozcanhan G, Zijlstra S, Lindenbergh A, Sijen T (2016) Prevalence of human cell material: DNA and RNA profiling of public and private objects and after activity scenarios. Forensic Sci Int Genet 21:81–89. https://doi.org/10.1016/j.fsigen.2015.12.012
Breathnach M, Williams L, McKenna L, Moore E (2015) Probability of detection of DNA deposited by habitual wearer and/or the second individual who touched the garment. Forensic Sci Int Genet 20:53–60. https://doi.org/10.1016/j.fsigen.2015.10.001
Cale C, Earl M, Lathan K, Bush G (2015) Could secondary DNA transfer falsely place someone at the scene of a crime? J Forensic Sci 61(1):196–203. https://doi.org/10.1111/1556-4029.12894
Verdon T, Mitchell R, Van Oorschot R (2013) The influence of substrate on DNA transfer and extraction efficiency. Forensic Sci Int Genet 7(1):167–175. https://doi.org/10.1016/j.fsigen.2012.09.004
Ballantyne K, Poy A, Van Oorschot R (2013) Environmental DNA monitoring: beware of the transition to more sensitive typing methodologies. Australian J Forensic Sci 45(3):323–340. https://doi.org/10.1080/00450618.2013.788683
Fitzgerald v The Queen (2014) HCA 28
Gill P (2014) Misleading DNA evidence. Acad Press 1:1–19
Noel S, Lagace K, Rogic A, Granger D, Bourgoin S, Jolicoeur C, Seguin D (2016) DNA transfer during laundering may yield complete genetic profiles. Forensic Sci Int Genet 23:240–247. https://doi.org/10.1016/j.fsigen.2016.05.004
Crowe G, Moss D, Elliot D (2000) The effect of laundering on the detection of acid phosphatase and spermatozoa on cotton T-shirts. Can Soc Forensic Sci J 33(1):1–5. https://doi.org/10.1080/00085030.2000.10757498
Kafarowski E, Lyon A, Sloan M (1996) The retention and transfer of spermatozoa in clothing by machine washing. J Can Soc Forensic Sci 29:7–11
Jobin R, De Gouffe M (2003) The persistence of seminal constituents on panties after laundering. Significance to investigations of sexual assault. J Can Soc Forensic Sci 36:1–10
Kamphausen T, Fandel S, Gutmann J, Bajanowski T, Poetsch M (2015) Everything clean? Transfer of DNA traces between textiles in the washtub. Int J Legal Med 129(4):709–714. https://doi.org/10.1007/s00414-015-1203-5
Farmen R, Cortez P, Froyland E (2008) Spermatozoa recovered on laundered clothing. Forensic Sci Int Genet Suppl Ser 1:418–420
Voskoboinik L, Amiel M, Reshef A, Gafny R, Barash M (2017) Laundry in a washing machine as a mediator of secondary and tertiary DNA transfer. Int J Legal Med. https://doi.org/10.1007/s00414-017-1617-3
Taylor D, Biedermann A, Samie L, Pun K, Hicks T, Champod C (2017) Helping to distinguish primary from secondary transfer events for trace DNA. Forensic Sci Int Genet 28:155–177. https://doi.org/10.1016/j.fsigen.2017.02.008
Acknowledgments
We would like to thank all volunteers who participated in this study and New South Wales Health Pathology, Forensic and Analytical Science Service for supplying DNA testing resources and for the analyses of the DNA profiles.
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Ruan, T., Barash, M., Gunn, P. et al. Investigation of DNA transfer onto clothing during regular daily activities. Int J Legal Med 132, 1035–1042 (2018). https://doi.org/10.1007/s00414-017-1736-x
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DOI: https://doi.org/10.1007/s00414-017-1736-x