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Identification of pulmonary edema in forensic autopsy cases of fatal anaphylactic shock using Fourier transform infrared microspectroscopy

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Abstract

Anaphylaxis is a rapid allergic reaction that may cause sudden death. Currently, postmortem diagnosis of anaphylactic shock is sometimes difficult and often achieved through exclusion. The aim of our study was to investigate whether Fourier transform infrared (FTIR) microspectroscopy combined with pattern recognition methods would be complementary to traditional methods and provide a more accurate postmortem diagnosis of fatal anaphylactic shock. First, the results of spectral peak area analysis showed that the pulmonary edema fluid of the fatal anaphylactic shock group was richer in protein components than the control group, which included mechanical asphyxia, brain injury, and acute cardiac death. Subsequently, principle component analysis (PCA) was performed and showed that the anaphylactic shock group contained more turn and α-helix protein structures as well as less tyrosine-rich proteins than the control group. Ultimately, a partial least-square discriminant analysis (PLS-DA) model combined with a variables selection method called the genetic algorithm (GA) was built and demonstrated good separation between these two groups. This pilot study demonstrates that FTIR microspectroscopy has the potential to be an effective aid for postmortem diagnosis of fatal anaphylactic shock.

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Funding

This project was supported by grants from the National Natural Science Foundation of China (81730056, 81722027, 81471819, 81601645 and 81671869), the National Key R&D Program of China (2016YFC0800701), and the Science and Technology Committee of Shanghai Municipality (17DZ2273200 and 16DZ2290900).

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  1. Hancheng Lin and Yiwen Luo contributed equally to this work.

Authors and Affiliations

  1. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, Shanghai, 200063, China

    Hancheng Lin, Yiwen Luo, Lei Wang, Kaifei Deng, Qiran Sun & Ping Huang

  2. Department of Forensic Pathology, Xi’an Jiaotong University, Xi’an, 710061, China

    Hancheng Lin, Ruoxi Fang, Xin Wei, Shuai Zha & Zhenyuan Wang

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  1. Hancheng Lin
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  2. Yiwen Luo
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Correspondence to Zhenyuan Wang or Ping Huang.

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Lin, H., Luo, Y., Wang, L. et al. Identification of pulmonary edema in forensic autopsy cases of fatal anaphylactic shock using Fourier transform infrared microspectroscopy. Int J Legal Med 132, 477–486 (2018). https://doi.org/10.1007/s00414-017-1721-4

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