International Journal of Legal Medicine

, Volume 132, Issue 4, pp 1079–1082 | Cite as

Forensic features and phylogenetic analyses of Sichuan Han population via 23 autosomal STR loci included in the Huaxia Platinum System

  • Guanglin He
  • Mengge Wang
  • Jing Liu
  • Yiping Hou
  • Zheng Wang
Population Data


Short tandem repeats (STRs) are DNA regions with a variable number of repeated units (2–6 bp) and attractive to genetic application like forensic, population genetics, and anthropological studies. The Huaxia Platinum System allows the multiplex amplification of 23 autosomal STR loci and two gender determination loci. In the present study, genetic polymorphisms of these 23 autosomal STR loci were first obtained from 309 Sichuan Han individuals, southwest China. No evidence of deviation from the Hardy-Weinberg equilibrium (HWE) was identified. The combined match probability (CMP), combined power of discrimination (CPD), and combined power of exclusion (CPE) are 1.087 × 10−27, 0.999999999999999999999999999, and 0.9999999996 in the Sichuan Han Chinese population, respectively. The inter-population differentiation, PCA, MDS, and phylogenetic analysis revealed that Sichuan Han had a closer genetic relationship with neighboring Han Chinese populations. Our findings indicated that the 23 autosomal STR loci are polymorphic and informative in studied southwestern Chinese Han population and can be utilized as a powerful tool for forensic applications. In addition, population comparisons demonstrated that the genetic similarities widely existed among Han Chinese populations distributed in different administrative divisions, and genetic differences had existed between minorities (most prominently for Uyghur and Kazakh) and Han groups.


Short tandem repeat Genetic polymorphism Forensic genetics Huaxia Platinum System 



We would like to thank the volunteers who contributed samples for this study.

Funding information

This study was supported by grants from the National Key R&D Program of China (2016YFC0800703) and National Natural Science Foundation of China (81501635, 81330073).

Compliance with ethical standards

The humane and ethical research principles recommended by Sichuan University were followed in this study. All participants signed the written informed consents before sample collection. Our study design was approved by the medical ethics committee of Sichuan University.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Figure S1

The geographic position of the investigated population and reference populations. (JPEG 611 kb).

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High resolution image (TIFF 6561 kb).
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Figure S2

Principal component analysis based on 19 overlapped STR loci of our studied population and 26 reference populations. (JPEG 153 kb).

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High resolution image (TIFF 1507 kb).
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Figure S3

Multidimensional Scaling plots showed the genetic relationships between the investigated population (bold font and red) and 26 reference populations. All Han Chinese populations distributed in different regions just marked their region name in the MDS. (JPEG 129 kb)

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High resolution image (TIFF 1839 kb).
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Figure S4

The phylogenetic relationship among the studied population and 26 reference populations, which was constructed by the unweighted pair-group method with arithmetic averages (UPGMA) based on 19 overlapped STR loci in the Mega7.0 software. (JPEG 305 kb).

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High resolution image (TIFF 4609 kb).
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Table S1 (XLSX 74 kb).
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Table S2 (XLSX 12.5 kb).
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Table S3 (XLSX 11.4 kb).
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Table S6 (XLSX 16.3 kb).


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Institute of Forensic Medicine, West China School of Basic Science and Forensic MedicineSichuan UniversityChengduChina

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