Short tandem repeats (STRs) are DNA regions with a variable number of repeated units (2–6 bp) and attractive to genetic application like forensic, population genetics, and anthropological studies. The Huaxia Platinum System allows the multiplex amplification of 23 autosomal STR loci and two gender determination loci. In the present study, genetic polymorphisms of these 23 autosomal STR loci were first obtained from 309 Sichuan Han individuals, southwest China. No evidence of deviation from the Hardy-Weinberg equilibrium (HWE) was identified. The combined match probability (CMP), combined power of discrimination (CPD), and combined power of exclusion (CPE) are 1.087 × 10−27, 0.999999999999999999999999999, and 0.9999999996 in the Sichuan Han Chinese population, respectively. The inter-population differentiation, PCA, MDS, and phylogenetic analysis revealed that Sichuan Han had a closer genetic relationship with neighboring Han Chinese populations. Our findings indicated that the 23 autosomal STR loci are polymorphic and informative in studied southwestern Chinese Han population and can be utilized as a powerful tool for forensic applications. In addition, population comparisons demonstrated that the genetic similarities widely existed among Han Chinese populations distributed in different administrative divisions, and genetic differences had existed between minorities (most prominently for Uyghur and Kazakh) and Han groups.
Short tandem repeat Genetic polymorphism Forensic genetics Huaxia Platinum System
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We would like to thank the volunteers who contributed samples for this study.
This study was supported by grants from the National Key R&D Program of China (2016YFC0800703) and National Natural Science Foundation of China (81501635, 81330073).
Compliance with ethical standards
The humane and ethical research principles recommended by Sichuan University were followed in this study. All participants signed the written informed consents before sample collection. Our study design was approved by the medical ethics committee of Sichuan University.
Conflict of interest
The authors declare that they have no conflict of interest.
Multidimensional Scaling plots showed the genetic relationships between the investigated population (bold font and red) and 26 reference populations. All Han Chinese populations distributed in different regions just marked their region name in the MDS. (JPEG 129 kb)
The phylogenetic relationship among the studied population and 26 reference populations, which was constructed by the unweighted pair-group method with arithmetic averages (UPGMA) based on 19 overlapped STR loci in the Mega7.0 software. (JPEG 305 kb).
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