The X-chromosomal short tandem repeats (STRs) with more informative than autosomal STRs in some complicated biological relationships identification due to its specific mode of genetic transmission can be used as a complementary tool in forensic case practices. In this study, we presented the population genetic data of 19 X-STRs, consisting of DXS10174, DXS10075, DXS10079, DXS101, DXS10101, DXS10103, DXS10134, DXS10135, DXS10148, DXS10159, DXS10162, DXS10164, DXS6789, DXS6809, DXS7132, DXS7423, DXS7424, DXS8378, and HPRTB loci, in a sample of 235 individuals of Tibetan nationality from Sichuan province, Southwest China. All 19 X-STR loci were consistent with Hardy-Weinberg equilibrium. The results showed that the combined power of discrimination in females and males are 0.999999999999999999997 and 0.9999999999997, respectively. In addition, the mean paternity exclusion chances based on the formula of MECKrüger, MECKishida, and MECDesmarais as well as MECDesmarais Duo are 0.99999991, 0.9999999999924, 0.9999999999929, and 0.999999985, respectively. In summary, our findings suggested that the AGCU X19 kit can be considered to serve as a high polymorphic information tool for forensic identification and kinship testing in the Sichuan Tibetan population. Furthermore, population genetic structure investigation between Sichuan Tibetan population and other 19 populations using PCA, MDS, and phylogenetic tree illustrated that significant genetic difference was observed between the Sichuan Tibetan and Malay, as well as the Xinjiang Uyghur population.
X-STR Tibetan population Genetic diversity Forensic genetics
This is a preview of subscription content, log in to check access
The study was supported by the Sichuan Science and Technology Support Program (2014SZ0012).
G.H. and Y.L. wrote the manuscript, X.Z. and Y.Z. collected the samples, G.H., Y.L., X.Z., M. W. and H.L. conducted the experiment and analyzed the results, X.Z. modified the manuscript. J.W. conceived the experiment.
Compliance with ethical standards
Our experiments were approved by the Ethics Committee of Sichuan University, China.
Conflict of interest
The authors declare that they have no conflict of interests.
Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10(3):564–567CrossRefPubMedGoogle Scholar
Hundertmark T, Hering S, Edelmann J, Augustin C, Plate I, Szibor R (2008) The STR cluster DXS10148-DXS8378-DXS10135 provides a powerful tool for X-chromosomal haplotyping at Xp22. Int J Legal Med 122:489–492CrossRefPubMedGoogle Scholar
Laan M, VW E, Khusnutdinova M, Remm S (2005) Pääbo. (2005) X-chromosome as a marker for population history: linkage disequilibrium and haplotype study in Eurasian populations. Eur J Hum Genet 13:452–462CrossRefPubMedPubMedCentralGoogle Scholar
He G-L, Chen P-Y, Gao B et al (2017) Phylogenetic analysis among 27 Chinese populations and genetic polymorphisms of 20 autosomal STR loci in a Chinese Uyghur ethnic minority group. Aust J Forensic Sci. https://doi.org/10.1080/00450618.2016.1275793
He G, Wang M, Liu J, Hou Y, Wang Z (2017) Forensic features and phylogenetic analyses of Sichuan Han population via 23 autosomal STR loci included in the Huaxia Platinum System. Int J Legal MedGoogle Scholar