International Journal of Legal Medicine

, Volume 132, Issue 4, pp 967–973 | Cite as

Establishment of 11 linked X-STR loci within 1.1 Mb to assist with kinship testing

  • James Chun-I Lee
  • Chun-Yen Lin
  • Li-Chin Tsai
  • Yu-Jen Yu
  • Keng-Hsien Liao
  • Adrian Linacre
  • Hsing-Mei Hsieh
Original Article

Abstract

This report identifies and characterizes 10 novel short tandem repeat (STR) loci on the human X chromosome, all of which are within a range of 1.1 Mb. These newly characterized loci were developed to aid in kinship assignment when the X chromosome is specifically required. The repeat DNA sequences were identified initially using data in GenBank and are located immediately upstream and downstream from the previously described locus DXS6807. Only those loci with seven or more observed alleles were used for further study resulting in the identification of 10 new loci. The distance between each pair of loci ranged from 24,998 to 244,701 bp with an average of approximately 110.8 kb. The number of observed alleles ranged from 7 to 30 for these 10 loci with a polymorphic information content ranging from 0.593 to 0.930. The LOD score from a pairwise linkage study ranged from 4.40 to 23.73, indicating that these 11 loci were highly linked, as expected. In line with standard forensic practice, all 11 loci can be amplified in one multiplex reaction, and comprehensive allelic ladders for all the loci have been constructed. These newly established 11 linked STR loci on the human X chromosome were found to be highly polymorphic and have the potential to aid in kinship testing where the X chromosome loci currently plays a role.

Keywords

X-STR Kinship testing Linkage disequilibrium LOD score test Haplotype 

Notes

Acknowledgements

We thank the Ministry of Science and Technology of Taiwan who supported the study by the grant NSC97-2320-B-002-037-MY3 and the Department of Medical Research at the National Taiwan University Hospital (Taipei, Taiwan) for assistance with capillary electrophoresis.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

Human oral swabs used in this study were collected with informed consent after approval from the Institutional Review Board of National Taiwan University Hospital (IRB No. 201212148RIND). The studies have been performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Funding

This study was funded by the Ministry of Science and Technology in Taiwan (Grant No. NSC97-2320-B-002-037-MY3).

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • James Chun-I Lee
    • 1
  • Chun-Yen Lin
    • 2
  • Li-Chin Tsai
    • 3
  • Yu-Jen Yu
    • 1
  • Keng-Hsien Liao
    • 1
  • Adrian Linacre
    • 4
  • Hsing-Mei Hsieh
    • 3
  1. 1.Department of Forensic Medicine, College of MedicineNational Taiwan UniversityTaipeiTaiwan
  2. 2.Institute of Forensic Medicine, Ministry of JusticeNew Taipei CityTaiwan
  3. 3.Department of Forensic ScienceCentral Police UniversityTaoyuanTaiwan
  4. 4.School of Biological SciencesFlinders UniversityAdelaideAustralia

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