The analysis of pupal development period in Lucilia sericata (Diptera: Calliphoridae) forensically important insect
Forensic entomology is a study that insect evidence is used for the determination of postmortem interval (PMImin). While determining PMImin, the pupal period is given as the duration between the beginning of the pupal period and the stage of adult emergence. The pupal period constitutes approximately 50 % of the immature development, and with detailed examination it would be possible to present a much more accurate PMImin. In this study, the pupal period of Lucilia sericata (Meigen, 1826), which is one of the species used in PMImin estimation in forensic entomology, was investigated. Studies about the pupal period of development are considerably limited in the forensic entomology world. Stages and duration of the pupal period of Lucilia sericata were studied at temperatures of 20 °C, 25 °C, and 30 °C. For all temperatures, pupae specimens were dissected and photographed hourly. According to findings based on all three temperatures, it was determined that as the temperature increases, the duration of development shortens. Also, in addition to 11 pre-determined development stages, nine new development stages were identified.
KeywordsLucilia sericata Pupae Development PMImin Temperature
We would like to thank Assoc. Prof. Dr. Hilal Özdağ from the Ankara University Institute of Biotechnology for proofreading and corrections. We also would like to thank Res. Assist. Dr. Burcu Şabanoğlu and Res. Assist. Dr. Senem Firat, and appreciate their help.
Conflict of interest
The authors declare that they have no conflicts of interest.
- 1.Goff ML (2001) A fly for the prosecution: how insect evidence helps solve crimes. Harvard University Press, CambridgeGoogle Scholar
- 2.Şabanoğlu B (2007) Determination of Calliphoridae (Diptera) Fauna on Carrion and Morphological Studies in the Point of Systematic Aspect in Ankara Province. Dissertation, Hacettepe University, Institute of Graduate Studies in ScienceGoogle Scholar
- 6.Greenberg B, Kunich JC (2002) Entomology and the law: flies as forensic indicators. Cambridge University Press, Cambridge, p 306Google Scholar
- 11.Agrel IPS, Lundquist AM (1973) Physiological and biochemical changes during insect development. In: Rockstein M (ed) The physiology of insecta. Academic, New YorkGoogle Scholar
- 12.Finell N, Jarvilehto M (1983) Development of compound eyes of the blowfly Calliphora erythrocephala: changes in morphology and function during metamorphosis. Ann Zool Fenn 20:223–234Google Scholar
- 13.Brown KE (2012) Utility of the Calliphora vicina (Diptera: Calliphoridae) Pupal Stage for Providing Temporal Information for Death Investigations. Dissertation, University of PortsmouthGoogle Scholar
- 15.Ergil C (2012) The Analysis of Pupal Developments in Calliphora vomitoria (Linnaeus, 1758) and Chrysomya albiceps (Wieddemann, 1819) Species which Belong to Calliphoridae Family that is Among the Forensically Important Insect Groups. Dissertation, Hacettepe University, Institute of Graduate Studies in ScienceGoogle Scholar
- 16.Zumpt F (1965) Myiasis in man and animals in the old world. Butterworths, LondonGoogle Scholar
- 17.Catts EP, Haskell NH (1990) Entomology and death: a procedural guide, 1st edn. Joyce’s Print Shop, South Carolina, pp 1–182Google Scholar