Fig. 5 | Chromosoma

Fig. 5

From: Ectopic expression of pericentric HSATII RNA results in nuclear RNA accumulation, MeCP2 recruitment, and cell division defects

Fig. 5

Timeline of ectopic HSATII expression. HSATII RNA (green) is expressed transiently 24 h after transfection in multiple nuclear accumulations and some diffuse nuclear RNA. Following antibiotic selection for 2 weeks (14 days), HSATII RNA bodies form, which recruit MeCP2 (orange), and accumulate adjacent to the genomic integration site of the HSATII expression vector (purple). At this point, HSATII RNA is restricted to these nuclear bodies and little diffuse nuclear RNA is observed. Approximately 3 weeks after transfection (21 days), significant cell division defects are observed, including lagging chromosomes (LC) between dividing daughter cells and the formation of micronuclei (MN)

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