Fig. 4 | Chromosoma

Fig. 4

From: Ectopic expression of pericentric HSATII RNA results in nuclear RNA accumulation, MeCP2 recruitment, and cell division defects

Fig. 4

Ectopic expression of satellite RNA leads to cell division defects. (a) Representative images of HeLa cells transfected and stably expressing HSATII RNA for 3 weeks exhibiting cell division defects including chromatin bridges (CB), blebbing (Bleb), and the formation of micronuclei (MN), as demonstrated in the images in the top 3 panels (DAPI stained, greyscale). Arrows indicate the type of defect observed. Graphs depict percentages of total cells counted displaying these defects: chromatin bridges (bottom left), blebbing/MN (bottom middle), and total chromatin defects (bottom right) out of 500 total cells for each transfection vector used or control (untransfected) cells. (b) Representative images of Tig-1 normal fibroblast cells transfected and stably expressing HSATII RNA for 3 weeks displaying cell division defects (lagging chromosomes (LC), blebbing, MN) as indicated by arrows. Graphs depict percentages of cells displaying these defects: chromatin bridges (bottom left), blebbing/MN (bottom middle), and total chromatin defects (bottom right) out of 500 total cells for each transfection vector used (X axis). Frequencies of chromatin defects significantly (p<.05) different from empty vector transfected cells by Chi-square test are indicated with two asterisks (**). Frequencies of chromatin defects significantly (p<.01) different from empty vector transfected cells by Chi-square test are indicated with three asterisks (***). Untransfected control cells included for comparison. Scale bar, 5μm

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