Fig. 2 | Chromosoma

Fig. 2

From: Ectopic expression of pericentric HSATII RNA results in nuclear RNA accumulation, MeCP2 recruitment, and cell division defects

Fig. 2

Stable expression of satellite RNA results in focal nuclear accumulation of HSATII RNA in a subset of cells. (a) Following integration and neomycin selection for the expression construct (HSATII, αSAT, empty), a subset of HeLa (ac) and Tig-1 (df) nuclei exhibit satellite RNA expression by RNA FISH. While α-sat RNA (a, d) is primarily diffuse in the nucleus, HSATII RNA accumulates in distinct nuclear foci (b, e) in both HeLa and Tig-1 cells. Cells transfected with an empty vector have no satellite RNA expression detectable by RNA FISH (c, f). (g, i) Quantification of cells with HSATII nuclear expression 3 weeks following transfection in HeLa (g) and Tig-1 (i). (h, j) RT-qPCR of HSATII RNA in transfected cell lines 3 weeks following transfection. Data is shown normalized to ß-actin (housekeeping gene). Error bars show 95% confidence interval. Scale bar, 5μm

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