Abstract
Nuclear bodies are relatively immobile organelles. Here, we investigated the mechanisms underlying their movement using experimentally induced interphase prenucleolar bodies (iPNBs). Most iPNBs demonstrated constrained diffusion, exhibiting infrequent fusions with other iPNBs and nucleoli. Fusion events were actin-independent and appeared to be the consequence of stochastic collisions between iPNBs. Most iPNBs were surrounded by condensed chromatin, while fusing iPNBs were usually found in a single heterochromatin-delimited compartment (“cage”). The experimentally induced over-condensation of chromatin significantly decreased the frequency of iPNB fusion. Thus, the data obtained indicate that the mobility of nuclear bodies is restricted by heterochromatin.
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Acknowledgements
We are grateful to X.W. Wang for the EGFP-NPM1-expressing plasmid and to G.M. Wahl for the histone H2B-EGFP-expressing plasmid. The work of Y.R.M. and Y.V. was partially conducted in the frame of the Koltzov Institute of Developmental Biology government program of basic research №. 0108-2018-0004.
Funding
The work was supported by the Russian Science Foundation (the light and electron microscopy by the grant 18-14-00195 to E.V.S.; the image processing and trajectory analysis by the grant 17-11-01279 to D.V.S.). The confocal laser scanning microscopy was supported by the Moscow State University Development Program (PNR 5.13), the Russian Foundation for Basic Research (project 18-04-00130), and the President’s grant (MK-716.2018.4).
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Arifulin, E.A., Sorokin, D.V., Tvorogova, A.V. et al. Heterochromatin restricts the mobility of nuclear bodies. Chromosoma 127, 529–537 (2018). https://doi.org/10.1007/s00412-018-0683-8
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DOI: https://doi.org/10.1007/s00412-018-0683-8